Long Linyu, Hu Cheng, Liu Wenqi, Wu Can, Lu Lu, Yang Li, Wang Yunbing
National Engineering Research Center for Biomaterials, Chuanda-Jinbo Joint Research Center, Sichuan University, Chengdu, 610064, China.
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan-Jinbo Joint Research Center, Fudan University, Shanghai 200302, China.
Carbohydr Polym. 2022 Aug 1;289:119456. doi: 10.1016/j.carbpol.2022.119456. Epub 2022 Apr 4.
Herein, an injectable multifunctional hydrogel based on dopamine grafted hyaluronic acid and phenylboric acid grafted methylcellulose was fabricated for promoting the repair of diabetic wounds. The prepared hydrogel possessed multifunctional properties including rapid gelation time (less than 10 s), self-healing, high water absorbency, tissue adhesiveness and excellent antioxidant activity. After loading Ag nanoparticles and the recombinant humanized collagen type III with high affinity to cells, the hydrogel exhibited properties of pH-/HO-responsive drug release profile, promoting cell proliferation and ideal antibacterial activity. Moreover, the in vivo experimental results demonstrated the prepared hydrogel could significantly accelerate wound repair by enhancing the collagen deposition and granulation tissue regeneration, reducing the expression of CD68 and improving the production of Ki67 and CD31 simultaneously. In conclusion, these multifunctional injectable hydrogels possessed great potential in chronic wound dressings.
在此，制备了一种基于多巴胺接枝透明质酸和苯硼酸接枝甲基纤维素的可注射多功能水凝胶，用于促进糖尿病伤口的修复。所制备的水凝胶具有多种功能特性，包括快速凝胶化时间（小于10秒）、自愈性、高吸水性、组织粘附性和优异的抗氧化活性。负载银纳米颗粒和对细胞具有高亲和力的重组人III型胶原蛋白后，该水凝胶表现出pH/HO响应性药物释放特性、促进细胞增殖和理想的抗菌活性。此外，体内实验结果表明，所制备的水凝胶可通过增强胶原蛋白沉积和肉芽组织再生、降低CD68表达以及同时提高Ki67和CD31的产生，显著加速伤口修复。总之，这些多功能可注射水凝胶在慢性伤口敷料方面具有巨大潜力。
