Postdoctoral Mobile Station of Basic Medical Sciences, Hengyang Medical School, China; Innovation Center for Molecular Target New Drug Study & School of Pharmaceutical Science, University of South China, Hengyang 421001, Hunan, China.
Innovation Center for Molecular Target New Drug Study & School of Pharmaceutical Science, University of South China, Hengyang 421001, Hunan, China.
Int J Biol Macromol. 2023 Jul 1;242(Pt 2):124872. doi: 10.1016/j.ijbiomac.2023.124872. Epub 2023 May 20.
A hydrogel wound dressing with a single functionality fails to meet the requirements for successful clinical treatment of chronic diabetic wounds that generally possess complicated microenvironments. A multifunctional hydrogel is thus highly desirable for improved clinical treatment. For this purpose, we reported herein construction of an injectable nanocomposite hydrogel with self-healing and photo-thermal properties as an antibacterial adhesive via dynamic Michael addition reaction and electrostatic interactions among three building moieties, i.e., catechol and thiol-modified hyaluronic acid (HA-CA and HA-SH), poly(hexamethylene guanidine) (PHMG), and black phosphorus nanosheets (BPs). An optimized hydrogel formulation eliminated over 99.99 % of bacteria (E. coli and S. aureus) and exhibited a free radical scavenging capability >70 % as well as photo-thermal properties in addition to viscoelastic characteristics, degradation properties in vitro, good adhesion and self-adaptation capacity. Wound healing experiments in vivo further confirmed the better performance of the developed hydrogels than that of a commercially available dressing (Tegaderm™) in promoting the healing of infected chronic wounds by preventing wound infection, decreasing inflammation, supporting collagen deposition, facilitating angiogenesis, and improving granulation tissues formation in the wound sites. Overall, the HA-based injectable composite hydrogels developed herein represent promising multifunctional wound dressings for infected diabetic wound repair.
一种具有单一功能的水凝胶敷料无法满足成功治疗慢性糖尿病伤口的要求，因为慢性糖尿病伤口通常具有复杂的微环境。因此，多功能水凝胶对于改善临床治疗非常有必要。为此，我们通过动态迈克尔加成反应和三个构建部分（即儿茶酚和巯基修饰的透明质酸（HA-CA 和 HA-SH）、聚六亚甲基胍（PHMG）和黑磷纳米片（BPs）之间的静电相互作用，报道了一种可注射的纳米复合水凝胶的构建，该水凝胶具有自修复和光热性能，是一种抗菌胶粘剂。优化的水凝胶配方消除了超过 99.99%的细菌（大肠杆菌和金黄色葡萄球菌），并具有自由基清除能力>70%以及光热性能，此外还具有粘弹性、体外降解性能、良好的粘附性和自适应能力。体内伤口愈合实验进一步证实了与市售敷料（Tegaderm™）相比，开发的水凝胶在促进感染性慢性伤口愈合方面的性能更好，其通过防止伤口感染、减少炎症、支持胶原蛋白沉积、促进血管生成以及改善伤口部位的肉芽组织形成来实现。总体而言，本文开发的基于 HA 的可注射复合水凝胶是一种有前途的多功能感染性糖尿病伤口修复敷料。
