Department of Cosmetics, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing, China.
Beijing Key Laboratory of Plant Research and Development, Beijing, China.
Exp Dermatol. 2022 Jul;31(7):1089-1094. doi: 10.1111/exd.14583. Epub 2022 May 6.
Atopic dermatitis (AD) is a recurring allergic skin disease that has a high incidence. Orally applied Bifidobacteria ameliorate signs of irritated skin and enhance the skin barrier. The present study investigated the safety and efficacy of a topically used cell-free culture supernatant (CFS) from a Bifidobacterium infantis strain using in vitro evaluation methods. The results showed that CFS had strong free radical scavenging activity on DPPH, ABTS, ·OH and O -radicals. CFS treatment fundamentally reduced the intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) contents and improved the activities of antioxidant enzymes (CAT, SOD and GSH-Px) in H O -treated HaCaT cells. Notably, the upregulation of skin physical barrier gene (FLG, LOR, IVL, AQP3 and TGM1) expression and skin antimicrobial peptide gene (CAMP, hBD-2 and hBD-3) expression by CFS might contribute to skin barrier resistance. CFS was non-irritating to the skin and eyes. CFS from the Bifidobacterium infantis strain had strong antioxidant properties on the skin and strengthened skin barrier function, and it was safe for topical use.
特应性皮炎（AD）是一种反复发作的过敏性皮肤病，发病率很高。口服双歧杆菌可改善皮肤炎症症状，增强皮肤屏障功能。本研究采用体外评价方法，研究了一种来源于婴儿双歧杆菌的无细胞培养上清液（CFS）的安全性和功效。结果表明，CFS 对 DPPH、ABTS、·OH 和 O -自由基具有很强的清除自由基活性。CFS 处理可从根本上降低 H 2 O 2 处理的 HaCaT 细胞内活性氧（ROS）和丙二醛（MDA）含量，并提高抗氧化酶（CAT、SOD 和 GSH-Px）的活性。值得注意的是，CFS 上调皮肤物理屏障基因（FLG、LOR、IVL、AQP3 和 TGM1）和皮肤抗菌肽基因（CAMP、hBD-2 和 hBD-3）的表达可能有助于皮肤屏障抵抗。CFS 对皮肤和眼睛无刺激性。来源于婴儿双歧杆菌的 CFS 对皮肤具有很强的抗氧化作用，增强了皮肤屏障功能，可安全用于局部使用。
