Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, People's Republic of China.
Curr Microbiol. 2011 Apr;62(4):1097-103. doi: 10.1007/s00284-010-9827-7. Epub 2010 Dec 4.
Several studies reported the antioxidant activity of bifidobacteria using assays in vitro. In present study, the in vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 was investigated. Culture supernatant, intact cells, and intracellular cell-free extracts of B. animalis 01 were involved in this study. The antioxidant assays in vitro included lipid peroxidation assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydroxyl radical ((•)OH) assay and superoxide anion (O₂⁻) assay. The antioxidant assays in vivo were conducted using mice model. Activities of antioxidative enzymes, malondialdehyde (MDA) content in serums and livers of aging mice were evaluated. Monoamine oxidase (MAO) activity and lipofuscin level in brains of aging mice were also characterized. Results showed that culture supernatant, intact cells and intracellular cell-free extracts of B. animalis 01 could effectively scavenge free radicals, significantly enhance mice's activities of antioxidative enzymes and reduce mice's MDA content, lipofuscin level and MAO activity. Our results indicated that B. animalis 01 has the potential to be developed into a dietary antioxidant supplements.
几项研究使用体外试验报告了双歧杆菌的抗氧化活性。本研究旨在研究动物双歧杆菌 01 的体外和体内抗氧化活性。本研究涉及双歧杆菌 01 的培养上清液、完整细胞和细胞内无细胞提取物。体外抗氧化试验包括脂质过氧化试验、1,1-二苯基-2-苦基肼（DPPH）试验、羟基自由基（(•)OH）试验和超氧阴离子（O₂⁻）试验。体内抗氧化试验采用小鼠模型进行。评估了衰老小鼠血清和肝脏中抗氧化酶的活性、丙二醛（MDA）含量，还研究了衰老小鼠大脑中单胺氧化酶（MAO）活性和脂褐素水平。结果表明，双歧杆菌 01 的培养上清液、完整细胞和细胞内无细胞提取物能有效清除自由基，显著提高小鼠抗氧化酶的活性，降低 MDA 含量、脂褐素水平和 MAO 活性。我们的结果表明，动物双歧杆菌 01 有潜力开发成膳食抗氧化补充剂。
