Institute of Biology, Plant Sciences, NAWI Graz, University of Graz, 8010, Graz, Austria.
Agricultural Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, 2462, Martonvásár, Hungary.
Histochem Cell Biol. 2022 Sep;158(3):213-227. doi: 10.1007/s00418-022-02103-2. Epub 2022 Apr 29.
Plant ascorbate and glutathione metabolism counteracts oxidative stress mediated, for example, by excess light. In this review, we discuss the properties of immunocytochemistry and transmission electron microscopy, redox-sensitive dyes or probes and bright-field microscopy, confocal microscopy or fluorescence microscopy for the visualization and quantification of glutathione at the cellular or subcellular level in plants and the quantification of glutathione from isolated organelles. In previous studies, we showed that subcellular ascorbate and glutathione levels in Arabidopsis are affected by high light stress. The use of light-emitting diodes (LEDs) is gaining increasing importance in growing indoor crops and ornamental plants. A combination of different LED types allows custom-made combinations of wavelengths and prevents damage related to high photon flux rates. In this review we provide an overview on how different light spectra and light intensities affect glutathione metabolism at the cellular and subcellular levels in plants. Findings obtained in our most recent study demonstrate that both light intensity and spectrum significantly affected glutathione metabolism in wheat at the transcriptional level and caused genotype-specific reactions in the investigated Arabidopsis lines.
植物抗坏血酸和谷胱甘肽代谢可抵抗氧化应激，例如，过量的光。在这篇综述中，我们讨论了免疫细胞化学和透射电子显微镜、氧化还原敏感染料或探针和明场显微镜、共聚焦显微镜或荧光显微镜在植物细胞或亚细胞水平上可视化和定量谷胱甘肽以及从分离的细胞器中定量谷胱甘肽的特性。在以前的研究中，我们表明拟南芥中亚细胞抗坏血酸和谷胱甘肽的水平受到高光胁迫的影响。发光二极管 (LED) 的使用在室内作物和观赏植物的种植中变得越来越重要。不同类型的 LED 的组合允许定制波长组合，并防止与高光子通量率相关的损坏。在这篇综述中，我们概述了不同的光谱和光强如何影响植物细胞和亚细胞水平的谷胱甘肽代谢。我们最近的研究结果表明，光强和光谱都显著影响了小麦在转录水平上的谷胱甘肽代谢，并导致所研究的拟南芥系中出现基因型特异性反应。
