将上胚层干细胞移植到上胚层并进行全胚胎成像以揭示谱系潜能。
Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence.
作者信息
Osteil Pierre, Studdert Joshua B, Tam Patrick P L
机构信息
Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
Swiss Cancer Research Institute (ISREC), School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
出版信息
Methods Mol Biol. 2022;2490:269-279. doi: 10.1007/978-1-0716-2281-0_19.
Here we describe a method to engraft epiblast stem cells (EpiSC) into the epiblast of gastrulation-stage mouse embryo to test the lineage propensity acquired by the EpiSCs during in vitro culture under different signaling conditions. After dissection and grafting, the recipient embryos can be grown in whole-embryo culture for up to 48 h and the contribution of the EpiSC-derived cells to tissues in the recipient embryo is assessed by light sheet 3D microscopy.
在此，我们描述了一种将上胚层干细胞（EpiSC）植入原肠胚形成期小鼠胚胎上胚层的方法，以测试在不同信号条件下体外培养期间EpiSC获得的谱系倾向。解剖和移植后，受体胚胎可在全胚胎培养中生长长达48小时，并通过光片三维显微镜评估EpiSC来源的细胞对受体胚胎组织的贡献。
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