从一位双侧葡萄膜炎患者房水中检测出钩端螺旋体基因组。
Detection of Leptospirosis Genome from the Aqueous Humor of a Patient with Bilateral Uveitis.
机构信息
Proctor Foundation, University of California Francis I., San Francisco, California, USA.
Department of Ophthalmology, University of California, San Francisco, California, USA.
出版信息
Ocul Immunol Inflamm. 2023 Jul;31(5):1076-1078. doi: 10.1080/09273948.2022.2067779. Epub 2022 Apr 29.
BACKGROUND
Leptospira species are difficult to culture. Thus, when there is suspicion for an infectious etiology to uveitis, bacterial cultures may fail to identify Leptospira. We describe a case of leptospirosis-associated uveitis that evaded culture and molecular assays. DNA sequencing of the aqueous fluid showed the presence of Leptospira spp.
METHODS
Retrospective case review of clinical and laboratory features of a patient with ocular leptospirosis is presented.
RESULTS
DNA sequencing identified the genome of Leptospirosis spp. in the aqueous humor.
CONCLUSION
Metagenomic sequencing, by virtue of its unbiased nature, can be a helpful adjunctive test when a strong clinical suspicion for intraocular infection persists despite negative routine culture and molecular assays.
背景
钩端螺旋体属很难培养。因此，当怀疑葡萄膜炎的病因具有传染性时，细菌培养可能无法检测到钩端螺旋体。我们描述了一例与钩端螺旋体病相关的葡萄膜炎，该疾病逃避了培养和分子检测。眼内液的 DNA 测序显示存在钩端螺旋体属。
方法
对一例眼内钩端螺旋体病患者的临床和实验室特征进行回顾性病例分析。
结果
DNA 测序在房水中鉴定出钩端螺旋体属的基因组。
结论
宏基因组测序具有无偏倚的性质，当尽管常规培养和分子检测均为阴性，但仍强烈怀疑眼内感染时，它可以作为一种有用的辅助检测手段。
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