Thomas Joanne, Nguyen Nam V, Fashina Tolulope, Huang Ye, Yeh Steven, Conrady Christopher D
Medical College of Georgia, Augusta University, Augusta, GA, United States.
College of Medicine, University of Nebraska Medical Center, Omaha, NE, United States.
Front Ophthalmol (Lausanne). 2023 Mar 21;3:1132131. doi: 10.3389/fopht.2023.1132131. eCollection 2023.
Early diagnosis of infectious uveitis can lead to prompt initiation of treatment to minimize vision-threatening sequelae. As various infectious etiologies of uveitis share similar clinical features, advancements in polymerase chain reaction (PCR) and metagenomic next-generation sequencing (MDS) have shown significant promise in improving diagnostic capabilities. Various techniques of PCR, including real-time, multiplex, comprehensive, and broad-range, have increased the armamentarium for infectious uveitis diagnosis. Additionally, metagenomic deep sequencing technology has provided a methodology to identify causative pathogens as well as novel etiologies of uveitis. This review discusses the diagnostic tools available for infectious uveitis and highlights the advantages and disadvantages of the techniques.
感染性葡萄膜炎的早期诊断能够促使及时开始治疗，以将威胁视力的后遗症降至最低。由于葡萄膜炎的各种感染病因具有相似的临床特征，聚合酶链反应（PCR）和宏基因组下一代测序（MDS）的进展在提高诊断能力方面显示出巨大潜力。PCR的各种技术，包括实时、多重、综合和广谱技术，增加了感染性葡萄膜炎诊断的手段。此外，宏基因组深度测序技术提供了一种识别致病病原体以及葡萄膜炎新病因的方法。本文综述讨论了可用于感染性葡萄膜炎的诊断工具，并强调了这些技术的优缺点。
