pBR 322编码的RTEMβ-内酰胺酶与底物的相互作用。特定构象转变的证据。
Interaction of the pBR 322-coded RTEM beta-lactamase with substrates. Evidence for specific conformational transitions.
作者信息
Citri N, Zyk N
出版信息
Biochem J. 1982 Feb 1;201(2):425-7. doi: 10.1042/bj2010425.
Abstract
The rate of inactivation of RTEM-1 beta-lactamase by Pronase is accelerated by class A ('resistant') penicillins. Other substrates (class S penicillin and cephalosporins) protect against the inactivation. Cefoxitin, a semi-synthetic cephamycin, induces a more extensive, hysteretic response. In its presence the enzyme is inactivated by trypsin as well as by Pronase.
摘要
A类（“耐药”）青霉素可加速链霉蛋白酶对RTEM-1β-内酰胺酶的灭活速率。其他底物（S类青霉素和头孢菌素）则可防止其灭活。半合成头霉素头孢西丁会引发更广泛的滞后反应。在其存在的情况下，该酶不仅会被链霉蛋白酶灭活，还会被胰蛋白酶灭活。
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