Department of Regenerative Medicine, Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
Biomed Mater. 2022 May 17;17(4). doi: 10.1088/1748-605X/ac6bd8.
Membranes prepared for guided bone regeneration (GBR) signify valued resources, inhibiting fibrosis and assisting bone regenration. However, existing membranes lack bone regenerative capacity or adequate degradation profile. An alginate-casted polycaprolactone-gelatin--tricalcium phosphate dual membrane was fabricated by electrospinning and casting processes to enhance new bone formation under a GBR process. Porous membranes were synthesized with suitable hydrophilicity, swelling, and degradation behavior to confirm the compatibility of the product in the body. Furthermore, osteoblast-type cell toxicity and cell adhesion results showed that the electrospun membrane offered compatible environment to cells while the alginate sheet was found capable enough to supress the cellular attachment, but was a non-toxic material. Post-implantation, theoutcomes of the dual-layered membrane, showed appreciable bone formation. Significantly, osteoid islands had fused in the membrane group by eight weeks. The infiltration of fibrous tissues was blocked by the alginate membrane, and the ingrowth of new bone was enhanced. Immunocytochemical analysis indicated that the dual membrane could direct more proteins which control mineralization and convene osteoconductive properties of tissue-engineered bone grafts.
用于引导骨再生（GBR）的膜是有价值的资源，能抑制纤维化并促进骨再生。但是，现有的膜缺乏骨再生能力或适当的降解特性。通过静电纺丝和浇铸工艺制备了海藻酸盐铸造的聚己内酯-明胶-磷酸三钙双层膜，以增强 GBR 过程中的新骨形成。多孔膜具有合适的亲水性、溶胀性和降解行为，以确认产品在体内的相容性。此外，成骨细胞毒性和细胞黏附结果表明，电纺膜为细胞提供了相容的环境，而海藻酸盐片足以抑制细胞附着，但它是一种无毒材料。植入后，双层膜的结果显示出明显的骨形成。值得注意的是，在膜组中，骨样岛在八周内融合。纤维组织的渗透被海藻酸盐膜阻止，新骨的生长得到增强。免疫细胞化学分析表明，双层膜可以引导更多控制矿化和召集组织工程骨移植物的骨传导特性的蛋白质。
