Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, South Korea.
Department of Regenerative Medicine, Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, South Korea.
J Biomater Sci Polym Ed. 2023 Jan;34(1):18-34. doi: 10.1080/09205063.2022.2106647. Epub 2022 Aug 5.
Guided bone regeneration (GBR) membranes favor periodontal regrowth, but they still have certain limitations, such as improper biodegradation and poor mechanical property. To overcome these shortcomings, we have generated a unique multifunctional membrane. A polycaprolactone/gelatin/β-TCP and alginate/gelatin trilayered construction was fabricated through electrospinning and casting technology. The prepared membranes have suitable physicomechanical and properties to confirm the compatibility of the product in the body. Phase analysis, functional groups, surface microstructure, and contact angle were measured as basic characteristics. For a mechanical performance evaluation, the tensile strength at suturing point was measured through pullout tensile strength test, and it showed the suture capability of bi-layered membranes. Highest tensile strength for A75G25 was recorded with 2.9 ± 0.15 MPa with 105% strain. Further, the osteoblast and fibroblast-type cell toxicity results showed that the electrospun membrane offered compatible environment to cells while the alginate sheet was found to be sufficiently capable to suppress the cellular attachment while also being a nontoxic material. Post-implantation, according to the conclusions of the tri-layered membrane, there was appreciable bone formation. Compared to an implant without membrane covering, enhanced new bone formation can be identified after 8 weeks of implantation with P1G4β10 membranes-covered site.
引导骨再生（GBR）膜有利于牙周组织再生，但仍存在一定的局限性，如生物降解不当和机械性能差。为了克服这些缺点，我们开发了一种独特的多功能膜。通过静电纺丝和浇铸技术制备了聚己内酯/明胶/β-TCP 和藻酸盐/明胶三层结构。所制备的膜具有合适的物理机械性能，以确认产品在体内的兼容性。通过相分析、官能团、表面微观结构和接触角来测量其基本特性。为了评估机械性能，通过拔出拉伸强度测试测量缝合点处的拉伸强度，以显示双层膜的缝合能力。A75G25 的拉伸强度最高，为 2.9±0.15 MPa，应变率为 105%。此外，成骨细胞和纤维母细胞型细胞毒性结果表明，电纺膜为细胞提供了相容的环境，而藻酸盐片具有足够的抑制细胞附着的能力，同时也是一种无毒材料。植入后，根据三层膜的结论，有明显的骨形成。与没有膜覆盖的植入物相比，用 P1G4β10 膜覆盖的部位在植入 8 周后可以识别出增强的新骨形成。
