School of Biosciences, Division of Natural Sciences, University of Kent, Canterbury, CT2 7NJ, UK.
Arch Microbiol. 2022 Apr 30;204(5):289. doi: 10.1007/s00203-022-02906-w.
In recent years, much attention has been focused on the biogenesis, engineering and utilisation of outer membrane vesicles (OMVs) in Gram-negative bacteria in a range of environments and niches. While the precise mechanism of biogenesis is unknown, it is focused on the modification of the Gram-negative cell wall to facilitate blebbing at sites of weakness in and around the characteristically thin peptidoglycan layer within the periplasm. Here, we investigate the biogenesis of membrane vesicles (MVs) in the Gram-positive organism Streptomyces albus S4 (Seipke et al. J Bacteriol 193:4270-4271, 2011 and Fazal et al. Antonie Van Leeuwenhoek 113:511-520, 2020). The S. albus S4 strain is an antifungal (candicidin and antimycin) producing organism that was isolated from attine ants (Barke et al. BMC Biol 8:109, 2010). The biogenesis and characterisation of S. albus S4 MVs is demonstrated using the wild-type (WT) and mutant strains ΔantC (no antimycin production) ΔfscC (no candicidin production) and ΔantC ΔfscC (produces neither antimycin nor candicidin). Here, we have shown that the S. albus S4 strain produces MVs and that these are comprised of both specific protein profiles and secondary metabolites, with a clear demonstration of the ability to selectively package one antifungal (candicidin) but not the other (antimycin).
近年来，人们对外膜囊泡（OMVs）在多种环境和生态位中的革兰氏阴性细菌的生物发生、工程和利用给予了极大的关注。虽然生物发生的确切机制尚不清楚，但它主要集中在对革兰氏阴性细胞壁的修饰上，以促进在特征性薄肽聚糖层周围和内部的薄弱部位起泡。在这里，我们研究了革兰氏阳性菌白链霉菌 S4（Seipke 等人，J Bacteriol 193:4270-4271, 2011 和 Fazal 等人，Antonie Van Leeuwenhoek 113:511-520, 2020）中膜囊泡（MVs）的生物发生。白链霉菌 S4 菌株是一种抗真菌（坎地西丁和安替霉素）产生菌，它是从切叶蚁中分离出来的（Barke 等人，BMC Biol 8:109, 2010）。使用野生型（WT）和突变株 ΔantC（不产生安替霉素）、ΔfscC（不产生坎地西丁）和 ΔantC ΔfscC（既不产生安替霉素也不产生坎地西丁）来证明白链霉菌 S4 菌株的 MV 生物发生和特性。在这里，我们已经表明，白链霉菌 S4 菌株产生 MV，并且这些 MV 由特定的蛋白质谱和次级代谢物组成，清楚地表明了选择性包装一种抗真菌剂（坎地西丁）而不是另一种（安替霉素）的能力。
