Li Z C, Liu M Z, Fang Y M, Gong Z J, Wang X L, Chen J D, Li B S
Institute of Pathogenic Microbiology of Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510000, China Guangdong Workstation for Emerging Infectious Disease Control and Prevention, Chinese Academy of Medical Sciences, Guangzhou 510000, China.
Institute of Microbiology of Zhuhai Center for Disease Control and Prenvention,Zhuhai 519000,China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2022 Apr 6;56(4):427-432. doi: 10.3760/cma.j.cn112150-20210517-00478.
To identify and analyze two strains of in Guangdong Province by combining whole genome sequencing with traditional detection methods. The was isolated from Guangzhou in 2010 and Zhuhai in 2020 respectively. Isolates were identified by API Coryne strips and MALDI-TOF-MS. Genomic DNA was sequenced by using Illumina. The assembly was performed for each strain using CLC software. J Species WS online tool was used for average nucleoside homology identification, then and gene were detected by NCBI online analysis tool BLSATN. MEGA-X was used to build a wgSNP phylogenetic tree. GD-Guangzhou-2010 was Belfanti and GD-Zuhai-2020 was Gravis. ANIb between GD-Guangzhou-2010 and was 99.61%. ANI between GD-Zhuhai-2020 and was 97.64%. BLASTN results showed that the nitrate reduction gene and gene of GD-Guangzhou-2010 was negative, while GD-Zhuhai-2020 nitrate reduction gene was positive. There were two obvious clades in wgSNP phylogenetic tree. The first clades included all Mitis and Gravis types strains as well as GD-Zhuhai-2020. The second clades contained all isolates of , subsp. and GD-guangzhou-2010. Two non-toxic strains are successfully isolated and identified. The phylogenetic tree suggests that GD-Guangzhou-2010 and GD-Zhuhai-2020 are located in two different evolutionary branches.
通过将全基因组测序与传统检测方法相结合，对广东省的两株[细菌名称未给出]进行鉴定和分析。该[细菌名称未给出]分别于2010年从广州和2020年从珠海分离得到。分离株通过API棒状杆菌鉴定条和基质辅助激光解吸电离飞行时间质谱进行鉴定。使用Illumina对基因组DNA进行测序。使用CLC软件对每个菌株进行组装。使用J Species WS在线工具进行平均核苷同源性鉴定，然后通过NCBI在线分析工具BLSATN检测[相关基因未给出]和[相关基因未给出]基因。使用MEGA-X构建wgSNP系统发育树。GD-Guangzhou-2010为Belfanti型，GD-Zuhai-2020为Gravis型。GD-Guangzhou-2010与[对比菌株未给出]之间的ANIb为99.61%。GD-Zhuhai-2020与[对比菌株未给出]之间的ANI为97.64%。BLASTN结果显示，GD-Guangzhou-2010的硝酸盐还原基因[相关基因未给出]和[相关基因未给出]基因为阴性，而GD-Zhuhai-2020的硝酸盐还原基因[相关基因未给出]为阳性。wgSNP系统发育树中有两个明显的分支。第一个分支包括所有Mitis和Gravis类型的菌株以及GD-Zhuhai-2020。第二个分支包含所有[细菌名称未给出]、[亚种名称未给出]亚种和GD-guangzhou-2010的分离株。成功分离并鉴定出两株无毒[细菌名称未给出]菌株。系统发育树表明，GD-Guangzhou-2010和GD-Zhuhai-2020位于两个不同的进化分支中。
