香叶天竺葵水醇提取物对乙酰氨基酚诱导的肾毒性大鼠的保护作用。

Protective Effect of the Hydroalcoholic Extract of Pelargonium Graveolens L. on Rats with Acetaminophen- Induced Nephrotoxicity.

作者信息

Gholyaf Mahmoud, Asmarian Sheida, Akbarpour Bijan, Gholyaf Mohammadreza, Mohammadi Farshid

机构信息

Clinical Research Development Unit of Shahid Beheshti hospital, Hamadan University of Medical Sciences, Hamadan, Iran.

出版信息

Iran J Kidney Dis. 2022 Mar;16(2):108-114.

PMID:35489079

Abstract

INTRODUCTION

Geranium has various antioxidant, anti-inflammatory, and anti-microbial effects. Prescribing glutathione probably enhances the protective mechanisms of nephrons against oxidative stresses. This study aimed to evaluate the protective effect of geranium on acetaminophen-induced nephrotoxic rats.

METHODS

In the present study, 70 mice were divided into seven groups. In five groups (T1, T2, T3, T4, and T5), different doses of geranium were given by gavage to the mice for seven days, then on the 8th day, a high dose of acetaminophen was administered intraperitoneally. Group T5 only received geranium extract. The control group received neither acetaminophen nor the extract while the last group received only a toxic dose of acetaminophen. Twenty-four hours after the last drug administration, blood samples were taken to check the levels of uric acid, blood nitrogen, and creatinine. The data were analyzed in SPSS version 25. To investigate the between-group factors' effects, one-way ANOVA with Tukey's post hoc test was performed at the alpha level of < 0.05.

RESULTS

The differences between the levels of blood creatinine, urea, and uric acid were significant (P < .001) among the groups. The mean blood urea for groups T3 and T4 were similar, and they had a significant difference in comparison with the control group (P < .05). The mean creatinine levels were similar between T4, T5, and the control groups and were significantly different from the other groups (P < .05). Blood uric acid for groups T1 and T2 were similar to Group B and higher than the other groups (P < .05).

CONCLUSION

The results showed that by strengthening cell protection mechanisms against oxidative stress, geranium extract reduces the toxic effects of acetaminophen on mice's kidney function and thus ameliorates the damage. As a result, the geranium extract has no adverse effects on kidney function. DOI: 10.52547/ijkd.6679.

摘要

引言

天竺葵具有多种抗氧化、抗炎和抗菌作用。开具谷胱甘肽可能会增强肾单位对氧化应激的保护机制。本研究旨在评估天竺葵对乙酰氨基酚诱导的肾毒性大鼠的保护作用。

方法

在本研究中，70只小鼠被分为七组。在五组（T1、T2、T3、T4和T5）中，通过灌胃给予小鼠不同剂量的天竺葵，持续七天，然后在第8天，腹腔注射高剂量的乙酰氨基酚。T5组仅接受天竺葵提取物。对照组既未接受乙酰氨基酚也未接受提取物，而最后一组仅接受有毒剂量的乙酰氨基酚。在最后一次给药24小时后，采集血样以检查尿酸、血氮和肌酐水平。数据在SPSS 25版中进行分析。为了研究组间因素的影响，在α水平<0.05时进行了单因素方差分析和Tukey事后检验。