用于现场同时制备样品和测定加工肉制品中亚硝酸盐的糖精掺杂聚乙烯醇薄膜。

Griess-doped polyvinyl alcohol thin film for on-site simultaneous sample preparation and nitrite determination of processed meat products.

机构信息

Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120 Thailand.

出版信息

Food Chem. 2022 Sep 30;389:133085. doi: 10.1016/j.foodchem.2022.133085. Epub 2022 Apr 25.

DOI:10.1016/j.foodchem.2022.133085

PMID:35489258

Abstract

To facilitate on-site nitrite determination for processed meat products, Griess-doped polyvinyl alcohol film was synthesized in the bottom of a plastic tube for in-tube determination. The tube's aperture was used to control the sample dimensions. Each sample, cut into eight sectors, was subjected to nitrite extraction by water. Use of tap water or commercial drinking water vs. ultrapure water was associated with < 2% differences in nitrite levels. The use of film and digital image colorimetry showed a low limit of detection (12.6 ± 0.5 µg L), good precision (1.0%RSD, n = 5 days), and good accuracy (93.2 ± 3.5 to 108.5 ± 1.8%recovery). Using these methods, sodium nitrite concentrations in 700 processed meat products for sale in Phuket, Thailand, were found to range from 6.8 ± 0.2 to 113.6 ± 1.3 mg kg. These results showed no significant differences with the HPLC standard method (p > 0.05, n = 45).

摘要

为了方便现场测定加工肉制品中的亚硝酸盐，我们在塑料管的底部合成了掺杂有格里厄斯试剂的聚乙烯醇薄膜，用于管内测定。管的孔径用于控制样品的尺寸。每个样品切成八个扇形，用水进行亚硝酸盐提取。与超纯水相比，使用自来水或商业饮用水与亚硝酸盐水平的差异<2%。使用薄膜和数字图像比色法，检测限低（12.6±0.5µg L），精密度好（1.0%RSD，n=5 天），准确度好（93.2±3.5 至 108.5±1.8%回收率）。使用这些方法，我们发现泰国普吉岛销售的 700 种加工肉制品中的亚硝酸钠浓度范围为 6.8±0.2 至 113.6±1.3mg kg。这些结果与 HPLC 标准方法没有显著差异（p>0.05，n=45）。