Löwkvist B, Emanuelsson H, Persson L, Sundler F, Lundquist A, Heby O
Cell Tissue Res. 1987 Jan;247(1):75-84. doi: 10.1007/BF00216549.
The localization of ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis and thus in cell growth, was determined in the 4.5-day-old chick embryo, using two independent methods of analysis. ODC protein was identified by indirect immunofluorescence with a monospecific ODC antibody, and catalytically active ODC was identified by autoradiography with alpha-(5-3H) difluoromethylornithine. Both methods revealed a basically similar distribution of ODC within the embryo. Among the organs, the brain exhibited the highest ODC levels. ODC levels were also high in spinal cord, mesonephric tubules and heart. Similar levels, but confined to limited areas, were found in liver tissue, head mesenchyme, and the oral and pharyngeal regions. Organs that exhibited high ODC levels are all engaged in rapid growth, as well as in extensive tissue remodeling and differentiation.
使用两种独立的分析方法，在4.5日龄的鸡胚中确定了鸟氨酸脱羧酶（ODC）的定位，ODC是多胺生物合成以及细胞生长中的关键酶。通过用单特异性ODC抗体进行间接免疫荧光鉴定ODC蛋白，并用α-（5-³H）二氟甲基鸟氨酸进行放射自显影鉴定具有催化活性的ODC。两种方法都揭示了ODC在胚胎内的分布基本相似。在这些器官中，脑呈现出最高的ODC水平。脊髓、中肾小管和心脏中的ODC水平也很高。在肝组织、头部间充质以及口腔和咽部区域发现了相似水平，但局限于有限区域。呈现高ODC水平的器官都参与快速生长以及广泛的组织重塑和分化。
