Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Mycoplasma Research Department, Animal Health Research Institute, Agriculture Research Center, Giza 12618, Egypt.
Poult Sci. 2022 Jun;101(6):101884. doi: 10.1016/j.psj.2022.101884. Epub 2022 Mar 26.
Respiratory diseases inflicted by Mycoplasma gallisepticum (MG) and Ornithobacterium rhinotracheale (ORT) cause severe economic losses and great burden to the poultry industry worldwide. Therefore, the current study was planned to assess the efficacy of aivlosin alone or in combination with zinc oxide nanoparticles (ZnO-NPs) in the treatment of experimental MG and/or ORT infections in broilers. Moreover, we also aimed to evaluate the role of ZnO-NPs on aivlosin residues in broiler tissues. A total of 1,440 Cobb chicks were allocated into 6 groups. At 14 d of age, chickens of groups 1 and 3 were experimentally infected with MG intratracheally and 6 d later, chickens of groups 2 and 3 were infected occulonasaly with ORT. Groups 1, 2, and 3 were divided into 4 subgroups; birds in subgroups 1, 2, and 3 were treated with aivlosin (A), ZnO-NPs (Z), and A/Z, respectively, while those in subgroups 4 was left without treatments. Moreover, groups 4 and 5 were kept noninfected and treated with aivlosin alone or in combination with ZnO-NPs, respectively. Finally, group 6 was kept as a negative control. The current results showed that the recovery from respiratory diseases caused by MG and/or ORT infections was most successful after treatment with A/Z in combination. Consequently, clinical signs, mortality rates, postmortem lesions of the respiratory organs, histopathological lesions of liver, trachea and lung and tracheal MG and ORT counts were significantly (P < 0.05) reduced following A/Z treatment. Taken together, high performance liquid chromatography analysis revealed that ZnO-NPs decreased the aivlosin residues in liver, muscle and skin of healthy and infected chickens. Based on these results, it could be concluded that aivlosin/ZnO-NPs therapy is a valuable approach for controlling MG and/or ORT infections in boilers.
鸡败血支原体（MG）和滑液囊支原体（ORT）引起的呼吸道疾病在全球范围内给家禽业造成了严重的经济损失和巨大的负担。因此，本研究旨在评估泰妙菌素单独或联合氧化锌纳米粒子（ZnO-NPs）治疗实验性 MG 和/或 ORT 感染肉鸡的疗效。此外，我们还旨在评估 ZnO-NPs 对肉鸡组织中泰妙菌素残留的作用。将 1440 只科布小鸡分配到 6 组。在 14 日龄时，第 1 组和第 3 组鸡经气管内接种 MG 进行实验感染，6 天后，第 2 组和第 3 组鸡经眼部接种 ORT 进行感染。第 1 组、第 2 组和第 3 组分为 4 个亚组；亚组 1、2 和 3 中的鸟类分别用泰妙菌素（A）、氧化锌纳米粒子（Z）和 A/Z 治疗，而亚组 4 中的鸟类则不进行治疗。此外，第 4 组和第 5 组保持未感染并分别用泰妙菌素单独或联合氧化锌纳米粒子治疗，第 6 组保持为阴性对照。目前的结果表明，用 A/Z 联合治疗 MG 和/或 ORT 感染引起的呼吸道疾病的恢复最为成功。因此，在用 A/Z 治疗后，临床症状、死亡率、呼吸道器官的尸检病变、肝脏、气管和肺的组织病理学病变以及气管 MG 和 ORT 计数显著（P <0.05）降低。综合来看，高效液相色谱分析显示，氧化锌纳米粒子降低了健康和感染鸡的肝脏、肌肉和皮肤中的泰妙菌素残留。基于这些结果，可以得出结论，泰妙菌素/氧化锌纳米粒子治疗是控制肉鸡 MG 和/或 ORT 感染的一种有价值的方法。
