GMBP1-conjugated manganese oxide nanoplates for monitoring of gastric cancer MDR using magnetic resonance imaging.

Multidrug resistance (MDR) is a huge challenge for gastric cancer chemotherapy. Therefore, MDR accurate monitoring is of great significance for the treatment of gastric cancer. GMBP1, an extracellular internalization peptide, can target MDR gastric cancer cells through specific binding to GRP78, which is an MDR-related protein that is overexpressed in gastric cancer cells. Herein, we constructed GMBP1 conjugated MnO nanoplates (MnO@PEG-GMBP1 NPs) for monitoring of MDR gastric cancer through magnetic resonance imaging (MRI). The generated MnO@PEG-GMBP1 NPs had a size of about 11 nm and exhibited a good colloidal stability in PBS and in 10% FBS medium. Serial MRI studies in mice demonstrated that the magnetic resonance signal intensity, at the tumor site, reached a peak at 3 h after tail vein injection of MnO@PEG-GMBP1 NPs. The specific targeting ability of MDR gastric cancer cells (SGC7901/ADR) by MnO@PEG-GMBP1 NPs was authenticated , and by immunofluorescence analysis experiments. The systematic safety evaluation indicated that the toxicity of MnO@PEG-GMBP1 NPs in mice was negligible. Therefore, the GMBP1 conjugated MnO nanoplates can be clinically used for accurate imaging and monitoring of MDR gastric cancer.