Engineering Research Center of Molecular & Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, 710126, China.
Radiology Department, Ninth Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, 710054, China.
J Nanobiotechnology. 2022 May 23;20(1):240. doi: 10.1186/s12951-022-01441-6.
Postoperative chemotherapy for gastric cancer often causes multidrug resistance (MDR), which has serious consequences for therapeutic effects. Individualized treatment based on accurate monitoring of MDR will greatly improve patient survival.
In this article, a self-enhanced MnO nanoplatform (MPG NPs) was established, which can react with glutathione to produce Mn to enhance T1-weighted magnetic resonance imaging (MRI) and mediate in vivo real-time MDR monitoring. In vitro MRI results showed that MRI signals could be enhanced in the presence of hydrogen peroxide and glutathione and at acidic pH. In vivo MRI results indicated that MPG NPs could specifically target MDR cells, thereby realizing real-time monitoring of MDR in gastric cancer. Furthermore, MPG NPs have good chemodynamic activity, which can convert the endogenous hydrogen peroxide of tumor cells into highly toxic hydroxyl radical through Fenton-like reaction at acidic pH to play the role of chemodynamic therapy. In addition, MnO can significantly enhance the chemodynamic therapy effect because of its good photothermal conversion effect. Furthermore, in situ photothermal/chemodynamic synergistic therapy obtained remarkable results, the tumors of the mice in the synergistic therapy group gradually became smaller or even disappeared.
MPG NPs have good biocompatibility, providing a good nanoplatform for real-time monitoring and precise diagnosis and treatment of MDR in gastric cancer.
胃癌术后化疗常导致多药耐药（MDR），对治疗效果产生严重影响。基于 MDR 准确监测的个体化治疗将极大地提高患者的生存率。
本文构建了一种自增强的 MnO 纳米平台（MPG NPs），它可以与谷胱甘肽反应生成 Mn 以增强 T1 加权磁共振成像（MRI）并介导体内实时 MDR 监测。体外 MRI 结果表明，在存在过氧化氢和谷胱甘肽以及酸性 pH 值的情况下，MRI 信号可以增强。体内 MRI 结果表明，MPG NPs 可以特异性靶向 MDR 细胞，从而实现胃癌 MDR 的实时监测。此外，MPG NPs 具有良好的化学动力学活性，可通过 Fenton 样反应在酸性 pH 值下将细胞内的内源性过氧化氢转化为高毒性羟基自由基，发挥化学动力学治疗作用。此外，MnO 由于其良好的光热转换效果，显著增强了化学动力学治疗效果。此外，原位光热/化学动力学协同治疗取得了显著效果，协同治疗组小鼠的肿瘤逐渐缩小甚至消失。
MPG NPs 具有良好的生物相容性，为胃癌 MDR 的实时监测和精确诊断与治疗提供了良好的纳米平台。
