Ritu G, Veerasigamani Geetha, Ashraf Mohammed C, Singh Sankalp, Laheri Saniya, Colaco Stacy, Modi Deepak
Department of Medical Genetics, Craft Hospital and Research Center, Trissur District, Kerala, India.
Reproductive Medicine, Craft Hospital and Research Center, Trissur District, Kerala, India.
J Hum Reprod Sci. 2022 Jan-Mar;15(1):82-89. doi: 10.4103/jhrs.jhrs_103_21. Epub 2022 Mar 31.
In patients undergoing assisted reproduction, levels of mitochondrial DNA (mtDNA) in the trophectodermal cells of the developing blastocyst are suggested to be associated with its ability to implant. However, discrepancies exist regarding the use of mtDNA levels as a reliable biomarker to predict outcomes of assisted reproduction.
The aim of the study is to explore the association of trophectodermal mtDNA levels to determine blastocyst quality, implantation potential of blastocyst and clinical outcomes in couples who have undergone pre-implantation genetic testing for aneuploidy (PGT-A).
Private fertility centre.
Retrospective analysis.
We analysed mtDNA levels in the trophectodermal cells of 287 blastocysts from 61 couples undergoing PGT-A. The levels of mtDNA were estimated by next-generation sequencing method. mtDNA levels were correlated with maternal age, blastocyst morphology, ploidy status, implantation rates, miscarriage rate and live birth rate.
Linear regression and one-way ANOVA with Tukey's all column comparison test.
The trophectodermal mtDNA levels did not correlate with maternal age. There were no significant differences in their levels in grade 1 and grade 2 blastocysts. No significant differences were seen between mtDNA levels of implanted and non-implanted blastocysts or those blastocysts that resulted in miscarriage or live birth. However, significantly lower amounts of mtDNA were seen in euploid blastocysts as compared to that in aneuploid blastocysts.
mtDNA levels in the trophectodermal cells of the blastocyst do not associate with blastocyst quality (grade 1 and grade 2), implantation potential and clinical outcomes but can differentiate between aneuploid and euploid blastocysts. Our study does not support the use of trophectodermal mtDNA levels as a biomarker for blastocyst quality and predictor of clinical outcomes.
在接受辅助生殖的患者中，发育中的囊胚滋养外胚层细胞中的线粒体DNA（mtDNA）水平被认为与其着床能力有关。然而，关于将mtDNA水平用作预测辅助生殖结局的可靠生物标志物，仍存在差异。
本研究的目的是探讨滋养外胚层mtDNA水平与囊胚质量、囊胚着床潜力以及接受植入前非整倍体基因检测（PGT-A）的夫妇的临床结局之间的关联。
私立生育中心。
回顾性分析。
我们分析了61对接受PGT-A的夫妇的287个囊胚滋养外胚层细胞中的mtDNA水平。mtDNA水平通过下一代测序方法进行估算。mtDNA水平与产妇年龄、囊胚形态、倍性状态、着床率、流产率和活产率相关。
线性回归和带有Tukey全列比较检验的单因素方差分析。
滋养外胚层mtDNA水平与产妇年龄无关。1级和2级囊胚中其水平无显著差异。着床和未着床囊胚或导致流产和活产的囊胚的mtDNA水平之间无显著差异。然而，与非整倍体囊胚相比，整倍体囊胚中的mtDNA量显著更低。
囊胚滋养外胚层细胞中的mtDNA水平与囊胚质量（1级和2级）、着床潜力和临床结局无关，但可区分非整倍体和整倍体囊胚。我们的研究不支持将滋养外胚层mtDNA水平用作囊胚质量生物标志物和临床结局预测指标。
