Zhong Can, Jin Jian, Zhou Rongrong, Liu Hao, Xie Jing, Wan Dan, Xiao Shengen, Zhang Shuihan
Horticulture and Landscape College Hunan Agricultural University Changsha China.
Institute of Chinese Materia Medica Hunan Academy of Chinese Medicine Changsha China.
Ecol Evol. 2022 Apr 25;12(4):e8818. doi: 10.1002/ece3.8818. eCollection 2022 Apr.
Cordyceps is a large group of entomogenous, medicinally important fungi. In this study, we sequenced, assembled, and annotated the entire mitochondrial genome of , in addition to comparing it against other three complete cordyceps mitogenomes that were previously published. Comparative analysis indicated that the four complete mitogenomes are all composed of circular DNA molecules, although their sizes significantly differ due to high variability in intron and intergenic region sizes in the and . mitogenomes. All mitogenomes contain 14 conserved genes and two ribosomal RNA genes, but varying numbers of tRNA introns. The Ka/Ks ratios for all 14 PCGs and were all less than 1, indicating that these genes have been subject to purifying selection. Phylogenetic analysis was conducted using concatenated amino acid and nucleotide sequences of the 14 PCGs and using two different methods (Maximum Likelihood and Bayesian analysis), revealing highly supported relationships between . and other species, in addition to a close relationship with . . Further, the analyses indicated that and play important roles in population differentiation. These mitogenomes will allow further study of the population genetics, taxonomy, and evolutionary biology of medicinally important cordyceps species.
虫草是一大类具有重要药用价值的虫生真菌。在本研究中，我们对[具体虫草名称]的整个线粒体基因组进行了测序、组装和注释，此外还将其与之前发表的其他三个完整的虫草线粒体基因组进行了比较。比较分析表明，这四个完整的线粒体基因组均由环状DNA分子组成，尽管由于[具体虫草名称1]和[具体虫草名称2]线粒体基因组中内含子和基因间隔区大小的高度变异性，它们的大小存在显著差异。所有线粒体基因组都包含14个保守基因和两个核糖体RNA基因，但tRNA内含子的数量各不相同。所有14个蛋白质编码基因（PCGs）的Ka/Ks比值均小于1，表明这些基因受到了纯化选择。使用14个PCGs的串联氨基酸和核苷酸序列，通过两种不同方法（最大似然法和贝叶斯分析）进行了系统发育分析，揭示了[具体虫草名称]与其他虫草物种之间有高度支持的关系，此外还与[具体虫草名称3]关系密切。此外，分析表明[具体虫草名称1]和[具体虫草名称2]在种群分化中起重要作用。这些线粒体基因组将有助于进一步研究具有重要药用价值的虫草物种的群体遗传学、分类学和进化生物学。
