College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, PR China.
Institute of Biomedical Science, Shanxi University, Taiyuan, 030006, PR China.
Talanta. 2021 Sep 1;232:122423. doi: 10.1016/j.talanta.2021.122423. Epub 2021 Apr 22.
In this work, a colorimetric and fluorescent dual mode sensor based on lysosome-targeted CDs has been desirably implemented to identify Fe fluctuations in vitro and in vivo. By simple one-pot hydrothermal carbonization of dried field mint, yellow-fluorescent CDs were directly fabricated without the assistance of other reagents and hold exceptional stability, superior biocompatibility as well as ultra-low cytotoxicity. Results indicated that as-prepared CDs can provide a rapid, reliable, and highly selective recognition of Fe with a linear range of 0 μM-400 μM and a detection limit of 0.037 μM. Impressively, it was found that as-developed CDs can successfully target lysosome with high colocalization coefficient (0.85) and responds to fluctuations of Fe in living cells. Further, acquired CDs was ingeniously devoted to Escherichia coli imaging. Besides, obtained CDs was eventually utilized to track the variation of Fe in vivo system. A preliminary research expresses that as-synthesized CDs can function as an effective tool to detect Fe in vitro and in vivo and thus indicates the promising applicability for disease detection in physiology and pathology in the future.
在这项工作中，设计了一种基于溶酶体靶向 CDs 的比色和荧光双模式传感器，以体外和体内识别 Fe 波动。通过简单的一锅干燥薄荷的水热碳化，无需其他试剂的辅助，直接制备了黄色荧光 CDs，具有优异的稳定性、良好的生物相容性和超低的细胞毒性。结果表明，所制备的 CDs 可以快速、可靠、高度选择性地识别 Fe，线性范围为 0 μM-400 μM，检测限为 0.037 μM。令人印象深刻的是，开发的 CDs 可以成功靶向具有高共定位系数（0.85）的溶酶体，并响应活细胞中 Fe 的波动。此外，将获得的 CDs 巧妙地用于大肠杆菌成像。此外，还利用获得的 CDs 跟踪体内系统中 Fe 的变化。初步研究表明，合成的 CDs 可以作为体外和体内检测 Fe 的有效工具，因此预示着在未来的生理和病理疾病检测中具有广阔的应用前景。
