Correia Mário S P, Thapa Bhawana, Vujasinovic Miroslav, Löhr J-Matthias, Globisch Daniel
Department of Chemistry - BMC, Science for Life Laboratory, Uppsala University Box 599 SE-75124 Uppsala Sweden
Department for Digestive Diseases, Karolinska University Hospital Stockholm Sweden.
RSC Adv. 2021 Oct 28;11(55):34788-34794. doi: 10.1039/d1ra05994g. eCollection 2021 Oct 25.
Metabolic microbiome interaction with the human host has been linked to human physiology and disease development. The elucidation of this interspecies metabolite exchange will lead to identification of beneficial metabolites and disease modulators. Their discovery and quantitative analysis requires the development of specific tools and analysis of specific compound classes. Sulfated metabolites are considered a readout for the co-metabolism of the microbiome and their host. This compound class is part of the human phase II clearance process of xenobiotics and is the main focus in drug or doping metabolism and also includes dietary components and microbiome-derived compounds. Here, we report the targeted analysis of sulfated metabolites in plasma and urine samples in the same individuals to identify the core sulfatome and similarities between these two sample types. This analysis of 27 individuals led to the identification of the core sulfatome of 41 metabolites in plasma and urine samples as well as an age effect for 15 metabolites in both sample types.
代谢微生物群与人类宿主的相互作用已与人类生理学和疾病发展相关联。对这种种间代谢物交换的阐明将有助于识别有益代谢物和疾病调节因子。它们的发现和定量分析需要开发特定的工具并对特定化合物类别进行分析。硫酸化代谢物被认为是微生物群与其宿主共代谢的一个指标。这类化合物是人体对外源化合物进行II期清除过程的一部分，也是药物或兴奋剂代谢的主要关注点，还包括饮食成分和微生物群衍生的化合物。在此，我们报告了对同一批个体的血浆和尿液样本中硫酸化代谢物进行靶向分析，以确定核心硫酸化代谢组以及这两种样本类型之间的相似性。对27名个体的这项分析导致在血浆和尿液样本中鉴定出41种代谢物的核心硫酸化代谢组，以及在两种样本类型中15种代谢物的年龄效应。
