Kumamoto Takuma, Ohtaka-Maruyama Chiaki
Developmental Neuroscience Project, Department of Brain and Neurosciences, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Front Neurosci. 2022 Apr 12;16:876406. doi: 10.3389/fnins.2022.876406. eCollection 2022.
Visualizing the process of neural circuit formation during neurogenesis, using genetically modified animals or somatic transgenesis of exogenous plasmids, has become a key to decipher cortical development and evolution. In contrast to the establishment of transgenic animals, the designing and preparation of genes of interest into plasmids are simple and easy, dispensing with time-consuming germline modifications. These advantages have led to neuron labeling based on somatic transgenesis. In particular, mammalian expression plasmid, CRISPR-Cas9, and DNA transposon systems, have become widely used for neuronal visualization and functional analysis related to lineage labeling during cortical development. In this review, we discuss the advantages and limitations of these recently developed techniques.
利用基因改造动物或对外源质粒进行体细胞转基因来可视化神经发生过程中神经回路的形成，已成为解读皮层发育和进化的关键。与建立转基因动物不同，将感兴趣的基因设计并制备到质粒中既简单又容易，无需耗时的种系修饰。这些优势促成了基于体细胞转基因的神经元标记。特别是，哺乳动物表达质粒、CRISPR-Cas9和DNA转座子系统，已广泛用于与皮层发育过程中谱系标记相关的神经元可视化和功能分析。在本综述中，我们讨论了这些最新开发技术的优缺点。
