鸡和蛇胚胎电穿孔研究大脑前部发育的方案。

Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas & Universidad Miguel Hernández, Sant Joan d'Alacant, 03550 Alacant, Spain.

STAR Protoc. 2021 Jul 28;2(3):100692. doi: 10.1016/j.xpro.2021.100692. eCollection 2021 Sep 17.

electroporation has become a key technique to study genetic mechanisms of brain development. However, electroporation of the embryonic pallium in oviparous species, interesting for evolutionary studies but distinct from electroporation, is quite infrequent. Here, we detail the electroporation of the developing pallium in chick and snake embryos. This protocol allows gene manipulation through introducing exogenous DNA into brain progenitor cells and can be adapted to any type of gene manipulation of the embryonic telencephalon. For complete information on the use and execution of this protocol, please refer to Cárdenas et al. (2018).

电穿孔已成为研究大脑发育遗传机制的关键技术。然而，对于进化研究很有趣但与电穿孔不同的卵生动物胚胎大脑皮层的电穿孔却相当罕见。在这里，我们详细描述了鸡和蛇胚胎发育中的大脑皮层的电穿孔。该方案通过将外源 DNA 导入脑祖细胞来实现基因操作，并且可以适应于胚胎端脑的任何类型的基因操作。有关此方案的使用和执行的完整信息，请参见 Cárdenas 等人。（2018 年）。