Sun Yuyan, Wang Bin, Chen Wei, Wang Yanbo, Zhou Dongdong, Zhang Mengyang, Zhang Chongqing, Li Ruiyun, He Jing
College of Forestry, Gansu Agricultural University, Lanzhou 730070, China.
Wolfberry Harmless Cultivation Engineering Research Center of Gansu Province, Lanzhou 730070, China.
Antioxidants (Basel). 2024 Dec 15;13(12):1537. doi: 10.3390/antiox13121537.
Wolfberry () is a vital economic tree species in northwest China, but root rot caused by occurs frequently, which seriously endangers the quality and yield of wolfberry. In this study, potato glycoside alkaloids (PGAs), a plant-derived active substance, were used as materials to explore its inhibitory effect on . By analyzing the changes of reactive oxygen species (ROS) level, antioxidant capacity, and apoptosis, the role of PGAs-mediated oxidative stress in inducing apoptosis of was revealed. The findings suggest that PGAs treatment inhibited mycelium growth, reduced biomass and sporulation, and delayed spore germination in . The concentration for 50% of maximal effect (EC) was 1.85 mg/mL. PGAs treatment induced an increase in caspase-3 activity, disrupting the cell membrane of fungi. In addition, PGAs treatment activated NADH oxidase (NOX) and superoxide dismutase (SOD), promoted hydrogen peroxide (HO) and superoxide anion (O) accumulation, and decreased ascorbate peroxidase (APX), glutathione reductase (GR), and dehydroascorbate reductase (DHAR) activities as well as oxidized glutathione (GSSG), reduced glutathione (GSH), and electron donor NADPH content. In summary, PGAs has a strong inhibitory effect on , and its inhibitory effect may be related to the promotion of ROS accumulation by PGAs, causing the disorder of intracellular redox balance of fungi, the decrease of total antioxidant capacity, and finally the induction of apoptosis. This study provides a new insight into the antifungal mechanism of PGAs against .
枸杞是中国西北重要的经济树种，但由[未提及的病原体]引起的根腐病频发，严重危及枸杞的品质和产量。本研究以植物源活性物质马铃薯糖苷生物碱（PGAs）为材料，探究其对[未提及的病原体]的抑制作用。通过分析活性氧（ROS）水平、抗氧化能力和细胞凋亡的变化，揭示了PGAs介导的氧化应激在诱导[未提及的病原体]细胞凋亡中的作用。研究结果表明，PGAs处理抑制了[未提及的病原体]菌丝体生长，降低了生物量和产孢量，并延缓了孢子萌发。半数最大效应浓度（EC）为1.85mg/mL。PGAs处理导致caspase-3活性增加，破坏了真菌细胞膜。此外，PGAs处理激活了NADH氧化酶（NOX）和超氧化物歧化酶（SOD），促进了过氧化氢（HO）和超氧阴离子（O）的积累，并降低了抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）和脱氢抗坏血酸还原酶（DHAR）的活性以及氧化型谷胱甘肽（GSSG）、还原型谷胱甘肽（GSH）和电子供体NADPH的含量。综上所述，PGAs对[未提及的病原体]具有较强的抑制作用，其抑制作用可能与PGAs促进ROS积累，导致真菌细胞内氧化还原平衡紊乱、总抗氧化能力下降，最终诱导细胞凋亡有关。本研究为PGAs对[未提及的病原体]的抗真菌机制提供了新的见解。
