Meka Kedir Welela, Dukassa Dubiwak Abebe, Tofik Ahmed Ebsa
Department of Chemistry, College of Natural and Computational Sciences, Mettu University, Mettu, Ethiopia.
Division of Medical Biochemistry, Department of Biomedical Sciences, Institute of Health Sciences, Jimma University, Jimma, Ethiopia.
J Toxicol. 2022 Apr 21;2022:8440019. doi: 10.1155/2022/8440019. eCollection 2022.
The kidney is the organ most vulnerable to nephrotoxic drugs such as gentamicin. Nephrotoxicity is a rapid deterioration of kidney function due to various factors. Gentamicin causes nephrotoxicity, which was manifested by an increase in serum kidney biomarkers. is one of the ethnomedicinal plants used as traditional medicine for treating various ailments, including kidney disease in Ethiopian society. Thus, the aim of this study is to evaluate the nephroprotective effect of root extract on gentamicin-induced nephrotoxicity. Using maceration techniques, 100 g of dried plant powder was extracted in 1 L of ethanol. The physicochemical screening of plant extracts revealed the presence of flavonoids, phenols, tannins, saponins, and steroids. The nephroprotective activity of crude extract was evaluated on male Swiss albino mice. The crude ethanolic extract at 200 and 400 mg/kg doses showed strong nephroprotective effects by restoring biomarkers such as creatinine, uric acid, and blood urea nitrogen, which were damaged by gentamicin ( < 0.05) in a dose-dependent manner. The mice treated with higher doses (400 mg/kg) had a comparable nephroprotective effect compared to the positive control group (200 mg/kg silymarin; > 0.05). The histopathology of the control group showed normal glomeruli, normal parenchyma, distal convoluted, and no tubular damage. The toxicant-induced group showed damage to glomeruli and inflammatory infiltration. Therefore, root extract has a nephroprotective activity by retarding the gentamicin toxicity in male Swiss albino mice.
肾脏是对庆大霉素等肾毒性药物最敏感的器官。肾毒性是由多种因素导致的肾功能快速恶化。庆大霉素会引发肾毒性，表现为血清肾脏生物标志物升高。[植物名称]是埃塞俄比亚社会中用作传统药物治疗各种疾病（包括肾脏疾病）的民族药用植物之一。因此，本研究的目的是评估[植物名称]根提取物对庆大霉素诱导的肾毒性的肾保护作用。采用浸渍技术，将100克干燥的植物粉末用1升乙醇提取。植物提取物的理化筛选显示存在黄酮类、酚类、单宁、皂苷和甾体。在雄性瑞士白化小鼠身上评估了[植物名称]粗提物的肾保护活性。200和400毫克/千克剂量的粗乙醇提取物通过恢复肌酐、尿酸和血尿素氮等被庆大霉素破坏的生物标志物，以剂量依赖的方式显示出强大的肾保护作用（P<0.05）。与阳性对照组（200毫克/千克水飞蓟宾；P>0.05）相比，接受较高剂量（400毫克/千克）治疗的小鼠具有相当的肾保护作用。对照组的组织病理学显示肾小球正常、实质正常、远曲小管正常且无肾小管损伤。毒物诱导组显示肾小球损伤和炎症浸润。因此，[植物名称]根提取物通过减轻雄性瑞士白化小鼠的庆大霉素毒性而具有肾保护活性。
