Ouahhoud Sabir, Bencheikh Noureddine, Khoulati Amine, Kadda Salma, Mamri Samira, Ziani Anas, Baddaoui Sanae, Eddabbeh Fatima-Ezzahra, Elassri Soufiane, Lahmass Iliass, Benabbes Redouane, Addi Mohamed, Hano Christophe, Choukri Mohammed, Bennani Amal, Asehraou Abdeslam, Saalaoui Ennouamane
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Université Mohamed Premier, Oujda 60000, Morocco.
Laboratory of Improvement of Agricultural Production, Biotechnology, and Environment, Department of Biology, Faculty of Sciences, Université Mohamed Premier, Oujda 60000, Morocco.
Evid Based Complement Alternat Med. 2022 Oct 1;2022:7127037. doi: 10.1155/2022/7127037. eCollection 2022.
The most costly spice in the world, L. (), has been used for more than 3,000 years. It has various beneficial applications in a range of fields, including aromas, colorants, and medications, but its usefulness as a food flavoring and coloring ingredient is the highest. Large quantities of by-products from the processing of saffron are typically thrown as unwanted bio-residues. This study's goal was to assess and compare the nephroprotective effects of hydroethanolic extracts of stigmas, tepals, and leaves on gentamicin (GM)-induced nephrotoxicity in rats. For that, we used a biochemical and histological investigation to propose new pharmaceutical valorizations. Based on the biochemical and histological analyses, it is concluded that all the studied parts of showed a renoprotective effect. Markedly, tepals revealed the most significant reduction of relative liver weight ( < 0.05), water intake ( < 0.05), plasma creatinine ( < 0.01), plasma urea ( < 0.01), plasma uric acid ( < 0.05), urinary protein ( < 0.01) and albumin ( < 0.001), and renal malondialdehyde (MDA) ( < 0.001). In addition, tepals caused a significant increase in body weight ( < 0.05), urinary creatinine ( < 0.01), creatinine clearance ( < 0.05), and urinary urea ( < 0.05) compared with the gentamicin untreated (GM) group. This is confirmed by the histopathological study which shows that treatment with stigmas, tepals, and leaves preserved kidney morphology at the glomerular and tubular cell level. The studied extracts exhibit good recovery potential for nephrotoxicity induced by gentamicin. In order to create potent dietary supplements or phytomedicines, it would also be very interesting to confirm these actions through clinical research.
世界上最昂贵的香料藏红花已被使用了3000多年。它在一系列领域有多种有益应用，包括香料、色素和药物，但作为食品调味和着色成分的用途最为突出。藏红花加工过程中产生的大量副产品通常作为无用的生物残渣被丢弃。本研究的目的是评估和比较藏红花柱头、花被片和叶子的水乙醇提取物对庆大霉素（GM）诱导的大鼠肾毒性的肾保护作用。为此，我们采用生化和组织学研究方法来提出新的药物价值评估。基于生化和组织学分析，得出结论：藏红花所有被研究的部位均显示出肾脏保护作用。值得注意的是，花被片显示出相对肝脏重量（P<0.05）、饮水量（P<0.05）、血浆肌酐（P<0.01）、血浆尿素（P<0.01）、血浆尿酸（P<0.05）、尿蛋白（P<0.01）和白蛋白（P<0.001）以及肾丙二醛（MDA）（P<0.001）的最显著降低。此外，与未用庆大霉素处理（GM）的组相比，藏红花花被片使体重（P<0.05）、尿肌酐（P<0.01）、肌酐清除率（P<0.05）和尿尿素（P<0.05）显著增加。组织病理学研究证实了这一点，该研究表明用柱头、花被片和叶子进行治疗在肾小球和肾小管细胞水平上保留了肾脏形态。所研究的提取物对庆大霉素诱导的肾毒性具有良好的恢复潜力。为了开发有效的膳食补充剂或植物药，通过临床研究来证实这些作用也将非常有意义。
