Frantz J M, Gebhardt B M, Reidy J J, McDonald M B
Lions Eye Research Laboratories, Louisiana State University Medical Center School of Medicine, New Orleans.
Refract Corneal Surg. 1991 Mar-Apr;7(2):141-5.
The purpose of this study was to compare the survival of epikeratophakia tissue lenses prepared with cryolathing and lyophilization (frozen lenses) and without (fresh lenses) in donor-sensitized recipients with vascularized corneas. Fresh lenses placed in vascularized corneas of immune recipients were subjected to immune attack. Frozen lenses placed in vascularized corneas of immunized recipients did not elicit an immune reaction. Neither fresh nor frozen lenses elicited immune reactions in nonvascularized corneas of immune recipients or in nonvascularized, nonimmune recipients. These results indicate that although the fresh lenses are more antigenic than the lenses in which the cells have been killed by freezing and lyophilization, the fresh lenses prepared using the BKS-1000 technique containing living stromal keratocytes are not likely to stimulate allograft immune reactions in unsensitized patients with avascular graft sites.
本研究的目的是比较在有血管化角膜的供体致敏受者中，用冷冻切割和冻干法制备的角膜表层镜片移植组织（冷冻镜片）与未用该方法制备的（新鲜镜片）的存活情况。置于免疫受者血管化角膜中的新鲜镜片会受到免疫攻击。置于免疫受者血管化角膜中的冷冻镜片不会引发免疫反应。新鲜镜片和冷冻镜片在免疫受者的无血管化角膜中或在无血管化、非免疫受者中均未引发免疫反应。这些结果表明，尽管新鲜镜片比经冷冻和冻干使细胞死亡的镜片具有更强的抗原性，但使用含有活的基质角膜细胞的BKS - 1000技术制备的新鲜镜片，不太可能在无血管移植部位的未致敏患者中刺激同种异体移植免疫反应。
