Nanotechnology Innovation Centre, Advanced Material Division, Mintek, 200 Malibongwe Drive, Private Bag X 3015, Johannesburg, Gauteng, South Africa.
SensorLab, Department of Chemistry, University of the Western Cape, Robert Sobukwe Rd, Bellvile, P.O. Box X75, Cape Town, 7535, South Africa.
Appl Biochem Biotechnol. 2023 May;195(5):3425-3455. doi: 10.1007/s12010-022-04277-w. Epub 2023 Jan 4.
17β-estradiol is used as a growth and fertility stimulant in the agronomic sector to induce fertility and manipulate reproductive characteristics in animals. However, unintended or unregulated distribution and exposure to even significant low levels of 17β-estradiol estrogen have detrimental health implication that can lead to reproductive abnormalities and even cancer. This could have severe effect on the ecosystem imbalance, food safety, to such a degree that its health impact necessitates rapid methods to probe for its prevalence and occurrence in the environment. Herein a simple, robust, sensitive and once-off use electrochemical biosensor to detect 17β-estradiol is developed, using 3-mercaptopropionic acid capped zinc selenide quantum dots trapped within the polyaniline (PANI) framework structure. The biosensor's interaction with the substrate was based on the capability of the hemeprotein, horseradish peroxidase (HRP) enzyme (i.e., baroreceptor) to alternatively catalyze phenolic alcohols. The biosensor displayed a significantly low limit of detection limit (LOD) of value 0.2 × 10 M towards 17β-estradiol. The Mechaelis-Menten constant (K) with the magnitude of 0.64 × 10 M was obtained; this indicates an outstanding affinity of the biosensing films towards 17β-estradiol. Subsequently, the developed biosensor was able to accurately and efficiently measure successive concentrations of 17β-estradiol from 0.2 × 10 to 4 × 10 M. The fabricated biosensor showed good selectivity towards 17β-estradiol compared to the other estrogenic endocrine-disrupting compounds such as estrone (E1), ethnylstradiol (EE2), and estriol (E3). The biosensor was capable of detecting 17β-estradiol in spiked tap water samples with good recoveries, thus affirming its potential to be applied for real electro-analysis of 17β-estradiol in treated wastewater.
17β-雌二醇在农业领域用作生长和生育刺激物，以诱导生育并操纵动物的生殖特征。然而，即使是低水平的 17β-雌二醇雌激素的意外或不受管制的分布和暴露也会对健康造成不利影响，导致生殖异常甚至癌症。这可能对生态系统平衡、食品安全产生严重影响，以至于其健康影响需要快速方法来探测其在环境中的存在和发生。本文开发了一种简单、稳健、灵敏且一次性使用的电化学生物传感器，用于检测 17β-雌二醇，该传感器使用 3-巯基丙酸封端的锌硒量子点捕获在聚苯胺（PANI）框架结构内。生物传感器与底物的相互作用基于血红素蛋白，辣根过氧化物酶（HRP）酶（即，压力感受器）交替催化酚醇的能力。生物传感器对 17β-雌二醇的检测限（LOD）值低至 0.2×10-7 M。获得的米氏常数（K）值为 0.64×10-7 M；这表明生物传感膜对 17β-雌二醇具有出色的亲和力。随后，开发的生物传感器能够准确高效地测量 17β-雌二醇从 0.2×10-7 到 4×10-7 M 的连续浓度。与其他雌激素内分泌干扰化合物（如雌酮（E1）、雌二醇（EE2）和雌三醇（E3））相比，该生物传感器对 17β-雌二醇表现出良好的选择性。该生物传感器能够检测自来水中 17β-雌二醇的加标样品，回收率良好，因此肯定了其在处理废水中实际分析 17β-雌二醇的潜力。
