评估人类脑胶质瘤增殖细胞沿模拟脑侵袭轨迹的线性微图案迁移的方案。

IFOM - the Firc Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy.

STAR Protoc. 2022 Apr 18;3(2):101331. doi: 10.1016/j.xpro.2022.101331. eCollection 2022 Jun 17.

Glioblastoma (GBM) cells invade the brain by following linear structures like blood vessel walls and white matter tracts by using specific motility modes. In this protocol, we describe two micropatterning techniques allowing recapitulation of these linear tracks : micro-contact printing and deep UV photolithography. We also detail how to maintain, transfect, and prepare human glioma propagating cells (hGPCs) for migration assays on linear tracks, followed by image acquisition and analysis, to measure key parameters of their motility. For complete details on the use and execution of this protocol, please refer to Monzo et al. (2016) and Monzo et al. (2021a).

胶质母细胞瘤（GBM）细胞通过使用特定的运动模式，沿着血管壁和白质束等线性结构浸润大脑。在本方案中，我们描述了两种微图案化技术，可重现这些线性轨迹：微接触印刷和深紫外光刻。我们还详细介绍了如何维持、转染和准备人神经胶质瘤增殖细胞（hGPCs），以便在线性轨迹上进行迁移分析，然后进行图像采集和分析，以测量其运动的关键参数。有关该方案使用和执行的完整详细信息，请参见 Monzo 等人（2016 年）和 Monzo 等人（2021a）。