QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Royal Brisbane and Women's Hospital, Brisbane, Australia.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.
从患者肿瘤组织中培养的低传代、无血清细胞系是神经胶质瘤 (GBM) 生物学临床前研究和细胞研究的金标准，但仍广泛使用根深蒂固、代表性差的细胞系模型，这影响了许多 GBM 研究的意义。我们认为，提供适当大小、有意义描述的参考集以及使用这些资源的适当工具，将促进对这些关键资源的更大采用。因此，我们提出了一个经过精心挑选的 12 种易于使用、遗传多样化、致瘤性、患者来源、低传代、无血清细胞系的面板，代表了 IDH 野生型 GBM 的分子亚型谱，以及它们详细的表型特征，外加一套用于生物发光/荧光标记、基因表达和 CRISPR/Cas9 介导的基因失活的定制慢病毒质粒。细胞系和所有伴随的数据都可以通过一个网站 Q-Cell（qimrberghofer.edu.au/q-cell/）轻松访问，所有质粒都可以从 Addgene 获得。这些资源对于寻求易于使用、特征良好、临床相关的 GBM 金标准模型的研究人员来说应该是有价值的。
