Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast.

Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-step protocol for the utilization of a CID system in live-cell microscopy experiments of budding yeast endocytosis. While focusing on the study of endocytosis, this protocol framework is adaptable to the study of other cellular processes in . For complete details on the use and execution of this protocol, please refer to Lamb et al. (2021).