In situ synthesis of Co-doped MoS nanosheet for enhanced mimicking peroxidase activity.

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To enhance the catalytic activity of two-dimensional layered materials as versatile materials, the modification of transition metal dichalcogenide nanosheets such as MoS by doping with heteroatoms has drawn great interests. However, few reports are available on the study of the enzyme-like activity of doped MoS. In this study, a facile in situ hydrothermal method for the preparation of various ultrathin transition metals (Fe, Cu, Co, Mn, and Ni) doped MoS nanosheets has been reported. Through the density functional theory (DFT) and steady-state kinetic analysis, the Co-doped MoS nanosheets exhibited the highest peroxidase-like catalytic activity among them. Furthermore, a typical colorimetric assay for HO was presented based on the catalytic oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to a blue product (oxTMB) by Co-MoS. The proposed colorimetric method showed excellent tolerance under extreme conditions and a broad linear range from 0.0005 to 25 mM for HO determination. Concerning the practical application, in situ detection of HO generated from SiHa cells was also fulfilled, fully confirming the great practicability of the proposed method in biosensing fields.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s10853-022-07201-z.