Iron/cobalt co-doped boron quantum dots as nanozymes with peroxidase-like activities and the nanozyme-involved cascade catalysis system for ratiometric fluorescence and dual-mode visual detection of glutamate.

To further explore the boron-involved nanomaterials toward efficient applications in chemo/bio sensing and detection fields, this work reports facile preparation of the emerging iron/cobalt co-doped boron quantum dots (Fe/Co@BQDs) that were explored as new artificial nanozymes for ratiometric fluorescence (FL) and visual detection of glutamate (Glu). In the presence of glutamate oxidase (GLOD), Glu was oxidized to produce HO, and then the HO was catalyzed by Fe/Co@ BQDs nanozymes to produce hydroxyl radical (•OH). Afterwards, the •OH induced FL quenching responses of rhodamine B (RhB) and Fe/Co@BQDs. Therefore, a new nanozyme-assisted cascade catalysis platform was explored, consisting of Fe/Co@BQDs, GLOD, and RhB. The platform was successfully used for ratiometric FL sensing of Glu and liquid/solid dual-channel FL visual semi-quantitative detection of Glu. The platform exhibits a board linear detection range of 1-500 µM, a low limit of detection of 0.3 µM, highly selective ratiometric FL responses on Glu over potential interferents, and high-performance practical detection of Glu in biological samples. Experimental results verify high peroxidase-like activities of Fe/Co@BQDs that enable efficient applications for unique enzymatic reactions and nanozyme-involved cascade catalysis reactions. The platform can facilitate further development of other types of metal-doped nanomaterials with natural biological enzyme-like activities and their promising applications, especially chemo/bio sensing, bioimaging and therapeutics at the levels of living cells and small animals.