Bisson L F, Neigeborn L, Carlson M, Fraenkel D G
J Bacteriol. 1987 Apr;169(4):1656-62. doi: 10.1128/jb.169.4.1656-1662.1987.
Glucose uptake mutants have not been previously obtained in Saccharomyces cerevisiae, possibly because there seem to be at least two transport systems, of low and high affinities. We showed that snf3 (sucrose nonfermenting) mutants did not express high-affinity glucose uptake. Furthermore, their growth was completely impaired on low concentrations of glucose in the presence of antimycin A (which blocks respiration). Several genes which complemented the original snf3 gene were obtained on multicopy plasmids. Some of them, as well as plasmid-carried SNF3 itself, conferred a substantial increase in high-affinity glucose uptake in both snf3 and wild-type hosts. The effects of glucose on the expression of such a plasmid-determined high-affinity uptake resembled those in the wild type. Other genes complementing snf3 seemed to cause an increase in low-affinity glucose uptake. We suggest that SNF3 may function specifically in high-affinity glucose uptake, which is needed under some conditions of growth on low glucose concentrations. SNF3 itself or the other complementing genes may specify components of the glucose uptake system.
以前尚未在酿酒酵母中获得葡萄糖摄取突变体，这可能是因为似乎至少存在两种具有低亲和力和高亲和力的转运系统。我们发现，snf3（蔗糖不发酵）突变体不表达高亲和力葡萄糖摄取。此外，在抗霉素A（阻断呼吸作用）存在的情况下，它们在低浓度葡萄糖上的生长完全受损。在多拷贝质粒上获得了几个与原始snf3基因互补的基因。其中一些基因，以及携带质粒的SNF3本身，在snf3和野生型宿主中都使高亲和力葡萄糖摄取大幅增加。葡萄糖对这种由质粒决定的高亲和力摄取表达的影响与野生型相似。其他与snf3互补的基因似乎会导致低亲和力葡萄糖摄取增加。我们认为，SNF3可能在高亲和力葡萄糖摄取中具有特定功能，这在低葡萄糖浓度下的某些生长条件下是必需的。SNF3本身或其他互补基因可能指定了葡萄糖摄取系统的组成部分。
