• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自黄色瘤胃球菌的纤维素酶基因在大肠杆菌中的克隆与表达

Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens.

作者信息

Barros M E, Thomson J A

出版信息

J Bacteriol. 1987 Apr;169(4):1760-2. doi: 10.1128/jb.169.4.1760-1762.1987.

DOI:10.1128/jb.169.4.1760-1762.1987
PMID:3549703
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212014/
Abstract

An endoglucanase gene of Ruminococcus flavefaciens FD1 was cloned on the vector pEcoR251 to form the recombinant plasmid pMEB200. The cloned endoglucanase gene showed carboxymethylcellulase enzyme activity but no degradation of Avicel (FMC Corp., Philadelphia, Pa.) or filter paper. Carboxymethylcellulase activity was found during the late-exponential-growth phase and accumulated in the periplasmic fraction. Enzyme production was not subject to catabolite repression by glucose.

摘要

将黄化瘤胃球菌FD1的一种内切葡聚糖酶基因克隆到载体pEcoR251上，构建重组质粒pMEB200。克隆的内切葡聚糖酶基因具有羧甲基纤维素酶活性，但对微晶纤维素（FMC公司，宾夕法尼亚州费城）或滤纸无降解作用。羧甲基纤维素酶活性在指数生长后期出现，并在周质部分积累。酶的产生不受葡萄糖的分解代谢物阻遏。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/212014/7def0b9ebcd8/jbacter00194-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/212014/83ac4b470fbc/jbacter00194-0406-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/212014/7def0b9ebcd8/jbacter00194-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/212014/83ac4b470fbc/jbacter00194-0406-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d981/212014/7def0b9ebcd8/jbacter00194-0407-a.jpg

相似文献

1
Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens.来自黄色瘤胃球菌的纤维素酶基因在大肠杆菌中的克隆与表达
J Bacteriol. 1987 Apr;169(4):1760-2. doi: 10.1128/jb.169.4.1760-1762.1987.
2
Modification of an endo-1,4-beta-glucanase from Ruminococcus albus by gene truncation.
Ann N Y Acad Sci. 1990;613:366-70. doi: 10.1111/j.1749-6632.1990.tb18180.x.
3
Carboxymethylcellulase and Avicelase activities from a cellulolytic Clostridium strain A11.来自纤维素分解梭菌菌株A11的羧甲基纤维素酶和微晶纤维素酶活性。
Curr Microbiol. 1995 May;30(5):305-12. doi: 10.1007/BF00295506.
4
Molecular study on cloned endoglucanase gene from rumen bacterium.
J Mol Microbiol Biotechnol. 2004;8(2):111-6. doi: 10.1159/000084566.
5
Cloning and expression of Clostridium acetobutylicum endoglucanase, cellobiase and amino acid biosynthesis genes in Escherichia coli.丙酮丁醇梭菌内切葡聚糖酶、纤维二糖酶及氨基酸生物合成基因在大肠杆菌中的克隆与表达
J Gen Microbiol. 1986 May;132(5):1367-72. doi: 10.1099/00221287-132-5-1367.
6
A cysteine desulphurase gene from the cellulolytic rumen anaerobe Ruminococcus flavefaciens.
Biochim Biophys Acta. 1998 Jul 28;1386(1):233-7. doi: 10.1016/s0167-4838(98)00062-4.
7
Expression of two xylanase genes from the rumen cellulolytic bacterium Ruminococcus flavefaciens 17 cloned in pUC13.克隆于pUC13的来自瘤胃纤维素分解菌黄色瘤胃球菌17的两个木聚糖酶基因的表达。
J Gen Microbiol. 1991 Jan;137(1):123-9. doi: 10.1099/00221287-137-1-123.
8
Cloning and expression in Escherichia coli of Thermotoga neapolitana genes coding for enzymes of carbohydrate substrate degradation.嗜热栖热放线菌中编码碳水化合物底物降解酶的基因在大肠杆菌中的克隆与表达
Biochem Biophys Res Commun. 1993 Aug 16;194(3):1359-64. doi: 10.1006/bbrc.1993.1974.
9
Mode of action, kinetic properties and physicochemical characterization of two different domains of a bifunctional (1-->4)-beta-D-xylanase from Ruminococcus flavefaciens expressed separately in Escherichia coli.在大肠杆菌中分别表达的来自黄化瘤胃球菌的双功能（1→4）-β-D-木聚糖酶两个不同结构域的作用模式、动力学特性及物理化学特征
Biochem J. 1993 Nov 15;296 ( Pt 1)(Pt 1):235-43. doi: 10.1042/bj2960235.
10
Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17.来自瘤胃厌氧菌黄色瘤胃球菌17的纤维素酶基因序列。
Mol Gen Genet. 1991 Aug;228(1-2):320-3. doi: 10.1007/BF00282484.

引用本文的文献

1
Soluble expression of recombinant active cellulase in E.coli using B.subtilis (natto strain) cellulase gene.利用枯草芽孢杆菌（纳豆芽孢杆菌菌株）纤维素酶基因在大肠杆菌中可溶性表达重组活性纤维素酶。
J Genet Eng Biotechnol. 2021 Jan 11;19(1):7. doi: 10.1186/s43141-020-00103-0.
2
Cloning a novel endo-1,4-β-D-glucanase gene from Trichoderma virens and heterologous expression in E. coli.从绿色木霉中克隆一个新的内切-1,4-β-D-葡聚糖酶基因并在大肠杆菌中进行异源表达。
AMB Express. 2016 Dec;6(1):108. doi: 10.1186/s13568-016-0282-0. Epub 2016 Nov 9.
3
Isolation and identification of cellulose-binding proteins from sheep rumen contents.

本文引用的文献

1
Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.刚果红-多糖相互作用在牛瘤胃纤维素分解菌计数与特性鉴定中的应用
Appl Environ Microbiol. 1982 Apr;43(4):777-80. doi: 10.1128/aem.43.4.777-780.1982.
2
Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria.可溶性碳水化合物对瘤胃细菌纯培养物消化纤维素的影响。
Appl Environ Microbiol. 1983 Sep;46(3):642-8. doi: 10.1128/aem.46.3.642-648.1983.
3
Simple, rapid, and quantitative release of periplasmic proteins by chloroform.
从绵羊瘤胃内容物中分离和鉴定纤维素结合蛋白。
Appl Environ Microbiol. 2009 Mar;75(6):1667-73. doi: 10.1128/AEM.01838-08. Epub 2009 Jan 16.
4
Molecular Cloning and Expression of Cellulase Genes from Ruminococcus albus 8 in Escherichia coli Bacteriophage lambda.在大肠杆菌噬菌体 λ中克隆和表达产黄瘤胃球菌的纤维素酶基因。
Appl Environ Microbiol. 1988 Jul;54(7):1752-5. doi: 10.1128/aem.54.7.1752-1755.1988.
5
Inhibitory Effects of Methylcellulose on Cellulose Degradation by Ruminococcus flavefaciens.甲基纤维素对黄瘤胃球菌纤维素降解的抑制作用。
Appl Environ Microbiol. 1988 Apr;54(4):890-7. doi: 10.1128/aem.54.4.890-897.1988.
6
Cloning, sequencing, and expression of a Eubacterium cellulosolvens 5 gene encoding an endoglucanase (Cel5A) with novel carbohydrate-binding modules, and properties of Cel5A.一种解纤维真杆菌（Eubacterium cellulosolvens）5基因的克隆、测序及表达，该基因编码一种具有新型碳水化合物结合模块的内切葡聚糖酶（Cel5A）以及Cel5A的特性
Appl Environ Microbiol. 2005 Oct;71(10):5787-93. doi: 10.1128/AEM.71.10.5787-5793.2005.
7
Cloning of the cellulase gene from Ruminococcus albus and its expression in Escherichia coli.从白色瘤胃球菌中克隆纤维素酶基因及其在大肠杆菌中的表达。
Appl Environ Microbiol. 1988 Jun;54(6):1511-5. doi: 10.1128/aem.54.6.1511-1515.1988.
8
Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.
Appl Environ Microbiol. 1989 May;55(5):1230-3. doi: 10.1128/aem.55.5.1230-1233.1989.
9
Cloning of an endo-1,4-beta-D-glucanase gene from Clostridium josui and its expression in Escherichia coli.从若芽孢梭菌中克隆endo-1,4-β-D-葡聚糖酶基因及其在大肠杆菌中的表达。
Appl Environ Microbiol. 1989 Sep;55(9):2399-402. doi: 10.1128/aem.55.9.2399-2402.1989.
10
Assessment of the endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1.黄化瘤胃球菌FD-1内切-1,4-β-葡聚糖酶组分的评估
Appl Environ Microbiol. 1990 Jun;56(6):1844-50. doi: 10.1128/aem.56.6.1844-1850.1990.
通过氯仿简单、快速且定量地释放周质蛋白。
J Bacteriol. 1984 Dec;160(3):1181-3. doi: 10.1128/jb.160.3.1181-1183.1984.
4
Rapid and efficient cosmid cloning.快速高效的黏粒克隆
Nucleic Acids Res. 1981 Jul 10;9(13):2989-98. doi: 10.1093/nar/9.13.2989.
5
Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria.用于瘤胃细菌非选择性计数和分离的不含瘤胃液的培养基。
Appl Microbiol. 1966 Sep;14(5):794-801. doi: 10.1128/am.14.5.794-801.1966.
6
Regulation of cellulase from Ruminococcus.来自瘤胃球菌的纤维素酶的调控
Can J Microbiol. 1972 Mar;18(3):347-53. doi: 10.1139/m72-053.
7
Microiodometric determination of beta-lactamase activity.β-内酰胺酶活性的微量碘量法测定
Antimicrob Agents Chemother. 1972 Feb;1(2):94-9. doi: 10.1128/AAC.1.2.94.
8
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
9
Properties and mode of action of cellulases.
Biotechnol Bioeng Symp. 1975(5):111-33.
10
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.在氯化钙中长时间孵育可提高大肠杆菌细胞的感受态。
Gene. 1979 May;6(1):23-8. doi: 10.1016/0378-1119(79)90082-9.