一种用于胰腺癌诊断的血清长链非编码RNA生物标志物的简单快速比色检测法。

A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer.

作者信息

Lou Ut Kei, Wong Chi Hin, Chen Yangchao

机构信息

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong Shatin NT Hong Kong

Shenzhen Research Institute, The Chinese University of Hong Kong Shenzhen 518087 China.

出版信息

RSC Adv. 2020 Feb 25;10(14):8087-8092. doi: 10.1039/c9ra07858d. eCollection 2020 Feb 24.

A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies.