Cahyadi Muhammad, Sukaryo Sukaryo, Dhiaurridho Mohammad Ilham, Bramastya Thoriq Aldri, Yanti Yuli, Riyanto Joko, Volkandari Slamet Diah, Sudrajad Pita
Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia.
Research Center for Biotechnology, Research Organization for Life Sciences, National Research and Innovation Agency (BRIN), Cibinong, Jawa Barat, Indonesia.
Vet World. 2022 Mar;15(3):782-788. doi: 10.14202/vetworld.2022.782-788. Epub 2022 Mar 30.
Pleomorphic adenoma gene 1 () encodes a multifunctional transcription factor that controls many genes and pathways and is associated with cattle body weight and measurements. This study aimed to evaluate the association between polymorphisms with body weight and measurements in Bali cattle.
A total of 87 Bali cattle, consisting of 48 bulls and 39 heifers at the Breeding Center for Bali Cattle, were used as the population in this study. Cattle were 2 years old and kept semi-intensively in the pasture. Phenotype data consisting of body weight, withers height, body length, chest girth, waist height, and chest depth were measured. Birth weight data were obtained from birth records, and weight gain, adjusted weaning weight, and yearling weight were calculated using formulas. Blood samples were taken from the jugular vein as much as 5 mL, and genomic DNA was isolated using the salting-out method. Polymerase chain reaction (PCR) was performed to amplify three target polymorphisms, namely, g.48308 C>T, g.32212 (19 bp indel), and g.45233 T>C. The presence of a 19 bp indel was determined by direct observation of the PCR product on a 2% agarose gel. Two other polymorphisms were detected by PCR-restriction fragment length polymorphism using the restriction endonuclease enzymes II and I. genotype and phenotype associations were analyzed using a general linear model.
The results showed that two of the target polymorphisms in did not vary. The DD genotype indicated by 123 bp of PCR product was the only genotype identified for g.32212 19 bp indel, and TT genotype was the only genotype found for g.45233 T>C single-nucleotide polymorphism (SNP). Conversely, g.48308 C>T SNP was found to be polymorphic. In addition, the g.48308 C>T polymorphism of was significantly associated with body length of Bali cattle. Cattle with the CC genotype had a greater body length than the other two genotypes.
The g.48308 C>T SNP in was associated with Bali cattle body length characteristics. This finding could be used as a basis for selecting Bali cattle based on body length characteristics.
多形性腺瘤基因1（）编码一种多功能转录因子，该因子控制许多基因和信号通路，且与牛的体重及体尺相关。本研究旨在评估巴厘牛中该基因多态性与体重及体尺之间的关联。
本研究以巴厘牛育种中心的87头巴厘牛为研究群体，其中包括48头公牛和39头母牛。牛龄为2岁，在牧场半集约化饲养。测量了包括体重、体高、体长、胸围、腰高和胸深在内的表型数据。出生体重数据来自出生记录，并使用公式计算体重增加量、校正断奶体重和周岁体重。从颈静脉采集5 mL血液样本，采用盐析法提取基因组DNA。进行聚合酶链反应（PCR）以扩增三个目标多态性位点，即g.48308 C>T、g.32212（19 bp插入/缺失）和g.45233 T>C。通过在2%琼脂糖凝胶上直接观察PCR产物来确定19 bp插入/缺失的存在情况。另外两个多态性位点通过使用限制性内切酶II和I的PCR-限制性片段长度多态性进行检测。使用一般线性模型分析该基因的基因型与表型之间的关联。
结果表明，该基因的三个目标多态性位点中有两个没有变异。PCR产物123 bp所指示的DD基因型是g.32212 19 bp插入/缺失唯一鉴定出的基因型，TT基因型是g.45233 T>C单核苷酸多态性（SNP）唯一发现的基因型。相反，g.48308 C>T SNP被发现具有多态性。此外，该基因的g.48308 C>T多态性与巴厘牛的体长显著相关。CC基因型的牛比其他两种基因型的牛体长更长。
该基因中的g.48308 C>T SNP与巴厘牛的体长特征相关。这一发现可为基于体长特征选择巴厘牛提供依据。
