Jakaria Jakaria, Kholijah Kholijah, Darwati Sri, Rahman Qonita, Daulay Winni Liani, Suhendro Ikhsan, Londra I Made, Ulum Mokhamad Fakhrul, Noor Ronny Rachman
Department of Animal Production and Technology, Faculty of Animal Science, IPB University (Bogor Agricultural University), Bogor 16680, Indonesia.
Agricultural Technology Study Center (BPTP), JL. By Pass Ngurah Rai, Pesanggaran, Denpasar Selatan 80222, Bali, Indonesia.
Vet World. 2023 Jun;16(6):1312-1318. doi: 10.14202/vetworld.2023.1312-1318. Epub 2023 Jun 13.
Coat color variations in cattle are known to be influenced by the melanocortin 1 receptor () and receptor tyrosine kinase () genes. The presence of coat color abnormalities, such as white spots and albinism, in Bali cattle was the focus of this study. This study aimed to identify single nucleotide polymorphisms (SNPs) in the coding region of and exons 2 and 3 of associated with coat color abnormalities in Bali cattle.
The study included the analysis of 48 Bali cattle, including 20 individuals with standard coat color, 15 with white spots, and 13 with albinism. Total DNA was extracted using a DNA Extraction Kit, and (coding region) and (exons 2 and 3) gene amplifications were analyzed using forward and reverse primers with polymerase chain reaction product lengths of 1071, 234, and 448 bp, respectively. The determination of and gene diversity was analyzed through direct sequencing. Melanocortin 1 receptor and gene sequence data were analyzed using BioEdit and MEGA6 to identify SNPs associated with standard and abnormal coat color phenotypes (white-spotted and albino) in Bali cattle.
No SNPs associated with coat color abnormalities were found in the coding region of and exons 2 and 3 of genes in Bali cattle. However, the intron two regions of contained the SNP g.70208534A>G, which showed a high degree of diversity. The AA genotype frequency was highest in albino Bali cattle, whereas the G allele frequency was highest and the A allele frequency was lowest in white-spotted Bali cattle.
The results indicated that standard, white-spotted, and albino coat colors in Bali cattle could not be distinguished by analyzing the and genes.
已知牛的毛色变异受黑素皮质素1受体（）和受体酪氨酸激酶（）基因影响。巴厘牛毛色异常（如白斑和白化）的存在是本研究的重点。本研究旨在鉴定与巴厘牛毛色异常相关的基因编码区以及基因外显子2和3中的单核苷酸多态性（SNP）。
本研究分析了48头巴厘牛，包括20头标准毛色个体、15头有白斑个体和13头白化个体。使用DNA提取试剂盒提取总DNA，并分别使用正向和反向引物通过聚合酶链反应分析基因（编码区）和基因（外显子2和3）的扩增，产物长度分别为1071、234和448 bp。通过直接测序分析基因和基因的多样性。使用BioEdit和MEGA6分析黑素皮质素1受体和基因序列数据，以鉴定与巴厘牛标准和异常毛色表型（白斑和白化）相关的SNP。
在巴厘牛基因的编码区以及基因的外显子2和3中未发现与毛色异常相关的SNP。然而，基因的内含子2区域包含SNP g.70208534A>G，其显示出高度的多样性。AA基因型频率在白化巴厘牛中最高，而G等位基因频率在有白斑的巴厘牛中最高，A等位基因频率最低。
结果表明，通过分析基因和基因无法区分巴厘牛的标准、白斑和白化毛色。
