Sepiol Caroline J, Yu Jaeju, Dhaubhadel Sangeeta
London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
Department of Biology, University of Western Ontario, London, ON, Canada.
Front Plant Sci. 2017 Dec 7;8:2073. doi: 10.3389/fpls.2017.02073. eCollection 2017.
Soybean ( [L.] Merr) is one of the main grain legumes worldwide. Soybean farmers lose billions of dollars' worth of yield annually due to root and stem rot disease caused by the oomycete . Many strategies have been developed to combat the disease, however, these methods have proven ineffective in the long term. A more cost effective and durable approach is to select a trait naturally found in soybean that can increase resistance. One such trait is the increased production of phytoalexin glyceollins in soybean. Glyceollins are isoflavonoids, synthesized via the legume-specific branch of general phenylpropanoid pathway. The first key enzyme exclusively involved in glyceollin synthesis is chalcone reductase (CHR) which coacts with chalcone synthase for the production of isoliquiritigenin, the precursor for glyceollin biosynthesis. Here we report the identification of 14 putative genes in soybean where 11 of them are predicted to be functional. Our results show that s display tissue-specific gene expression, and that only root-specific s are induced upon infection. Among 4 root-specific s, is located near a QTL that is linked to resistance suggesting as a novel locus for partial resistance that can be utilized for resistance breeding.
大豆（[L.] Merr）是全球主要的谷物豆类之一。由于卵菌引起的根腐病和茎腐病，大豆种植者每年损失价值数十亿美元的产量。人们已经开发了许多策略来对抗这种疾病，然而，这些方法从长远来看已被证明是无效的。一种更具成本效益和持久性的方法是选择大豆中天然存在的能够增强抗性的性状。其中一个这样的性状是大豆中植保素黄豆苷元产量的增加。黄豆苷元是异黄酮类化合物，通过通用苯丙烷途径中豆科植物特有的分支合成。唯一专门参与黄豆苷元合成的第一个关键酶是查尔酮还原酶（CHR），它与查尔酮合酶共同作用以产生异甘草素，这是黄豆苷元生物合成的前体。在此，我们报告了在大豆中鉴定出14个推定基因，其中11个预计具有功能。我们的结果表明，这些基因表现出组织特异性基因表达，并且只有根特异性基因在感染后被诱导。在4个根特异性基因中，一个位于与抗性相关的数量性状位点附近，表明该基因是一个可用于抗性育种的部分抗性新位点。
