Bai Guangjian, Ye Miaoyun, Yu Li, Yang Ming, Wang Yaqi, Chen Shaodan
Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Food Chem X. 2024 Jul 18;23:101671. doi: 10.1016/j.fochx.2024.101671. eCollection 2024 Oct 30.
var. (Longya lily) is an edible vegetable and medicinal plant with the effects of moistening lungs, relieving coughs, and removing phlegm. In this study, a homogenous mannoglucan LLP11 was purified from Longya lily using membrane ultrafiltration followed by ion exchange chromatography. The M of LLP11 was 12.0 kDa. LLP11 exhibited a backbone of →4)-α-D-Glc-(1 → and →4)-β-D-Man-(1 → with a branch of T-α-D-Glc-(1 → substituted at C-6 of →4,6)-α-D-Glc-(1→. During the simulated digestion, LLP11 remained indigestible to digestive enzymes. Furthermore, through its interaction with the gut microbiota, LLP11 was able to significantly boost and decrease the harmful bacteria , that was linked to pneumonia. Additionally, LLP11 promoted the growth of and and was utilized to produce acetic acid. Our findings introduced an alternative approach for the investigation of microbiota-targeted polysaccharides and underscored the potential of LLP11 as a prebiotic for supplementary treatment in respiratory diseases.
变种（龙牙百合）是一种具有润肺、止咳、祛痰功效的可食用蔬菜和药用植物。在本研究中，采用膜超滤和离子交换色谱法从龙牙百合中纯化出一种同质甘露葡聚糖LLP11。LLP11的分子量为12.0 kDa。LLP11呈现出→4)-α-D-葡萄糖-(1→和→4)-β-D-甘露糖-(1→的主链，以及在→4,6)-α-D-葡萄糖-(1→的C-6位被T-α-D-葡萄糖-(1→取代的分支。在模拟消化过程中，LLP11对消化酶仍具有抗性。此外，通过与肠道微生物群的相互作用，LLP11能够显著增加有益菌并减少与肺炎相关的有害菌。此外，LLP11促进了有益菌的生长，并被用于产生乙酸。我们的研究结果为研究针对微生物群的多糖提供了一种替代方法，并强调了LLP11作为呼吸道疾病辅助治疗益生元的潜力。
