Del Rosario Moguel Concha Deyanira, Martínez José Eduardo Borges, Velázquez Tzayhrí Guadalupe Gallardo, Martínez Cristian Jiménez, Ruiz Jorge Carlos Ruiz
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Av. Wilfrido Massieu Esq. Cda. Miguel Stampa S/N, Delegación Gustavo A. Madero C.P. 07738, Mexico.
Escuela de Nutrición, División de Ciencias de la Salud, Universidad Anáhuac-Mayab, Km 15.5 Carretera Mérida a Progreso, Int. Km 2 Carretera a Chablekal, Mérida, Yucatán C.P. 97310, Mexico.
Food Chem X. 2022 Jan 19;13:100219. doi: 10.1016/j.fochx.2022.100219. eCollection 2022 Mar 30.
During germination processes take place that modify the major components of the grain, such is the case of proteins that are hydrolyzed to generate peptides that can lead to the generation of bioactivity. The objective of the present work was to germinate grains of to evaluate the effect on the soluble protein content and the anti-inflammatory activity. The grains were subjected to 10 days of germination at 24 °C and relative humidity of 75%. Sprouts were lyophilized, milled, and phenolic compounds were extracted to avoid interferences. Soluble protein content varied significantly during the 10 days of germination. assays indicate that sprouts protein inhibits thermal denaturation of proteins, protease activity, and stabilize cell membranes. The IC50 values indicate that after germination the bioactivity increased between 1.4 and 3.5 times, with respect to the ungerminated grains. Results indicated that sprouts may constitute promising health-promoting foods.
在发芽过程中会发生改变谷物主要成分的变化,蛋白质水解产生可导致生物活性产生的肽的情况就是如此。本研究的目的是使谷物发芽,以评估其对可溶性蛋白质含量和抗炎活性的影响。将谷物在24℃和75%相对湿度下进行10天发芽处理。将芽苗冻干、研磨,并提取酚类化合物以避免干扰。在10天的发芽过程中,可溶性蛋白质含量有显著变化。实验表明,芽苗蛋白可抑制蛋白质的热变性、蛋白酶活性并稳定细胞膜。IC50值表明,发芽后生物活性相对于未发芽谷物提高了1.4至3.5倍。结果表明,芽苗可能构成有前景的健康促进食品。