Matsubara Teruhiko, Ogami Ayaka, Kori Haruka, Hashizume Mineo, Sato Toshinori
Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan.
Department of Industrial Chemistry, Tokyo University of Science, 12-1 Ichigayafunagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
ACS Appl Bio Mater. 2022 May 16;5(5):2130-2134. doi: 10.1021/acsabm.2c00276. Epub 2022 May 2.
The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.
血凝抑制（HAI）试验是全球流感监测中检测流感病毒（IFV）的方法之一，该方法使用新鲜制备的动物红细胞（RBC）。在此，我们证明了一种可预先通过化学方法制备的混合聚糖修饰的聚苯乙烯微粒能够在HAI试验中替代动物RBC。含有唾液酸和硫酸化乳糖部分的叠氮化物共轭聚糖混合物由用于IFV分离的犬肾传代细胞（MDCK）产生，然后使用点击化学固定在聚苯乙烯微粒表面。使用混合聚糖固定化微粒时，能够高灵敏度地检测人源血凝素（HA）和IFV。
