Dittmann Klaus H, Mayer Claus, Stephan Heribert, Mieth Christin, Bonin Michael, Lechmann Beat, Rodemann H Peter
Division of Radiation Biology, Dept of Radiation Oncology, Medical Faculty, Eberhard Karls University Tübingen, Roentgenweg 11, 72076, Tuebingen, Germany.
Neue Magnetodyn GmbH, Hermann-Oberth-Str. 9, 85640, Putzbrunn, Germany.
J Exp Orthop. 2022 May 2;9(1):39. doi: 10.1186/s40634-022-00477-9.
Molecular processes in primary osteoblasts were analyzed in response to magnetic and electric field exposure to examine its potential impact on bone healing.
Primary osteoblasts were exposed to a combination of a magnetic field and an additional electric field (EFMF) (20 Hz, 700 mV, 5 mT, continuous sinusoids) in vitro. mRNA- and protein-expressions were assessed during a time interval of 21 days and compared with expression data obtained from control osteoblasts.
We observed an autonomous osteoblast differentiation process in vitro under the chosen cultivation conditions. The initial proliferative phase was characterized by a constitutively high mRNA expression of extracellular matrix proteins. Concurrent EFMF exposure resulted in significanly increased cell proliferation (fold change: 1.25) and reduced mRNA-expressions of matrix components (0.5-0.75). The following reorganization of the extracellular matrix is prerequisite for matrix mineralization and is characterised by increased Ca deposition (1.44). On molecular level EFMF exposure led to a significant decreased thrombospondin 1 (THBS1) mRNA- (0.81) and protein- (0.54) expression, which in turn reduced the TGFß1-dependent mRNA- (0.68) and protein- (0.5) expression of transforming growth factor beta induced (ßIG-H3) significantly, an inhibitor of endochondral ossification. Consequently, EFMF exposure stimulated the expression of genes characteristic for endochondral ossification, such as collagen type 10, A1 (1.50), osteopontin (1.50) and acellular communication network factor 3 (NOV) (1.45).
In vitro exposure of osteoblasts to EFMF supports cell differentiation and induces gene- and protein-expression patterns characteristic for endochondral ossification during bone fracture healing in vivo.
分析原代成骨细胞在暴露于磁场和电场时的分子过程，以研究其对骨愈合的潜在影响。
将原代成骨细胞在体外暴露于磁场和附加电场的组合（EFMF）（20赫兹，700毫伏，5毫特斯拉，连续正弦波）。在21天的时间间隔内评估mRNA和蛋白质表达，并与从对照成骨细胞获得的表达数据进行比较。
在所选培养条件下，我们在体外观察到了自主的成骨细胞分化过程。初始增殖阶段的特征是细胞外基质蛋白的mRNA表达持续高水平。同时暴露于EFMF导致细胞增殖显著增加（倍数变化：1.25），基质成分的mRNA表达降低（0.5 - 0.75）。随后细胞外基质的重组是基质矿化的先决条件，其特征是钙沉积增加（1.44）。在分子水平上，EFMF暴露导致血小板反应蛋白1（THBS1）的mRNA（0.81）和蛋白质（0.54）表达显著降低，这反过来又显著降低了软骨内骨化抑制剂转化生长因子β诱导（βIG - H3）的TGFβ1依赖性mRNA（0.68）和蛋白质（0.5）表达。因此，EFMF暴露刺激了软骨内骨化特征性基因的表达，如10型胶原蛋白A1（1.50）、骨桥蛋白（1.50）和无细胞通讯网络因子3（NOV）（1.45）。
成骨细胞在体外暴露于EFMF可支持细胞分化，并在体内骨折愈合过程中诱导软骨内骨化特征性基因和蛋白质表达模式。
