中国西南地区腹泻型肠易激综合征与健康对照者肠道微生物群的显著差异
Significant Differences in Gut Microbiota Between Irritable Bowel Syndrome with Diarrhea and Healthy Controls in Southwest China.
作者信息
Yao Chengjiao, Li Yilin, Luo Lihong, Xie Fengjiao, Xiong Qin, Li Tinglin, Yang Chunrong, Feng Pei-Min
机构信息
Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610000, Sichuan, China.
Department of Geriatrics of the Affiliated Hospital, North Sichuan Medical College, Nanchong, Sichuan, China.
出版信息
Dig Dis Sci. 2023 Jan;68(1):106-127. doi: 10.1007/s10620-022-07500-0. Epub 2022 May 3.
BACKGROUND
Irritable bowel syndrome (IBS) is a heterogeneous disease, which is closely related to environmental factors and gut microbiota.
OBJECTIVE
To study gut microbiota in IBS-D of Han nationality in Southwest China and explore its relationship with environmental factors.
METHODS
One hundred and twenty cases of IBS-D and 63 cases of HCs were recruited; baseline data such as age, height, and weight were collected. HAMA, HAMD, IBS-SSS, IBS-QOL, and laboratory tests were performed. Feces were collected for 16S rDNA sequencing. Then, the differences of gut microbiota were analyzed and looked for biomarkers of each. FAPROTAX was used to predict the functional differences of gut microbiota. Spearman analysis was conducted between the phylum level and environmental factor.
RESULTS
There were significant differences in daily life between IBS-D and HCs, especially in the spicy taste. The scores of HAMA and HAMD, urea, and transaminase in IBS-D were significantly higher than those of HCs. The richness of gut microbiota in IBS-D was significantly lower than that of HCs, as well as the beta diversity, but not diversity. The biomarkers of IBS-D were Prevotella, Clostridiales, and Roseburia, and the biomarkers of HCs were Veillonellaceae, Bacteroides coprocola, and Bifidobacteriales. The functions of gut microbiota in IBS-D were significantly different from HCs. Correlation analysis showed that multiple gut microbiota were closely related to HAMA, IBS-SSS, IBS-QOL, inflammatory indexes, and liver enzymes.
CONCLUSION
There are significant differences in richness of gut microbiota, flora structure, and flora function between IBS-D and HCs in Southwest China. These differences may be closely related to environmental factors such as eating habits, living habits, and mental and psychological factors.
CLINICAL TRIAL REGISTRATION
The trial was registered and approved in China Clinical Trial Registry (Registration No. ChiCTR2100045751).
背景
肠易激综合征（IBS）是一种异质性疾病，与环境因素和肠道微生物群密切相关。
目的
研究中国西南地区汉族腹泻型肠易激综合征（IBS-D）患者的肠道微生物群，并探讨其与环境因素的关系。
方法
招募120例IBS-D患者和63例健康对照（HCs）；收集年龄、身高、体重等基线数据。进行汉密尔顿焦虑量表（HAMA）、汉密尔顿抑郁量表（HAMD）、IBS症状严重程度量表（IBS-SSS）、IBS生活质量量表（IBS-QOL）评估及实验室检查。采集粪便进行16S rDNA测序。然后，分析肠道微生物群的差异并寻找各自的生物标志物。使用FAPROTAX预测肠道微生物群的功能差异。在门水平与环境因素之间进行Spearman分析。
结果
IBS-D患者与HCs在日常生活方面存在显著差异，尤其是在辛辣口味方面。IBS-D患者的HAMA和HAMD评分、尿素及转氨酶水平显著高于HCs。IBS-D患者肠道微生物群的丰富度显著低于HCs，β多样性也是如此，但不是多样性。IBS-D的生物标志物是普雷沃氏菌属、梭菌目和罗斯氏菌属，HCs的生物标志物是韦荣氏菌科、粪拟杆菌和双歧杆菌目。IBS-D患者肠道微生物群的功能与HCs显著不同。相关性分析表明，多种肠道微生物群与HAMA、IBS-SSS、IBS-QOL、炎症指标和肝酶密切相关。
结论
中国西南地区IBS-D患者与HCs在肠道微生物群丰富度、菌群结构和菌群功能方面存在显著差异。这些差异可能与饮食习惯、生活习惯以及精神心理因素等环境因素密切相关。
临床试验注册
该试验在中国临床试验注册中心注册并获得批准（注册号：ChiCTR2100045751）。
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