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[提取物名称]的乙醇提取物可抑制肝癌细胞的增殖和迁移。 （你提供的原文中“suppresses liver cancer cell proliferation and migration”前缺少具体物质，这里补充了“[提取物名称]的”，请根据实际情况修改）

Ethanol extract of suppresses liver cancer cell proliferation and migration.

作者信息

Liu Hui, Liao Wanqin, Fan Lixia, Zheng Zhaoguang, Liu Dahai, Zhang Qing-Wen, Yang Anping, Liu Fang

机构信息

1Department of Basic Medicine and Biomedical Engineering, School of Stomatology and Medicine, Foshan University, Foshan, People's Republic of China.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao Sar, People's Republic of China.

出版信息

Chin Med. 2020 Jan 31;15:11. doi: 10.1186/s13020-020-0291-4. eCollection 2020.

DOI:10.1186/s13020-020-0291-4
PMID:32021647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6995237/
Abstract

BACKGROUND

belonging to the genus (Rubiaceae), is distributed throughout tropical and subtropical Asia. In this study, we evaluated for the first time the anti-proliferation and anti-migration effects of ethanol extract of (OPE) on HepG2 and SMMC-7721 cells, and explored the related mechanism.

METHODS

OPE was prepared by percolation with 95% ethanol and its main compounds were analyzed by HPLC-MS. The anti-proliferation effect of OPE was evaluated by the CCK-8 assay and colony formation assay. Cell cycle distribution, apoptosis, and reactive oxygen species (ROS) level were detected by flow cytometry. Migration and invasion abilities were detected by Transwell migration/invasion assays. The expression of correlated proteins was determined using western blotting.

RESULTS

A total of 5 tentative compounds were identified from OPE, including pumiloside, deoxypumiloside, camptothecin, aknadinine, and β-stigmasterol. OPE displayed strong cytostatic effects on HepG2 and SMMC-7721 cells. OPE induced G2/M phase cell cycle arrest, increased apoptosis, and augmented ROS production in these cell lines. In addition, OPE possessed a significant inhibition on cell migration and invasion by reduction of MMP-9 and MMP-2 expression. Moreover, OPE significantly suppressed the phosphorylation of p65.

CONCLUSIONS

Our data showed that OPE suppresses liver cancer cell proliferation and migration, which is possibly involved with the inhibition of the NF-κB pathway.

摘要

背景

（茜草科）属植物分布于亚洲热带和亚热带地区。在本研究中，我们首次评估了 乙醇提取物（OPE）对HepG2和SMMC - 7721细胞的抗增殖和抗迁移作用，并探讨了相关机制。

方法

采用95%乙醇渗漉法制备OPE，并通过HPLC - MS分析其主要成分。通过CCK - 8法和集落形成试验评估OPE的抗增殖作用。采用流式细胞术检测细胞周期分布、凋亡及活性氧（ROS）水平。通过Transwell迁移/侵袭试验检测迁移和侵袭能力。使用蛋白质印迹法测定相关蛋白的表达。

结果

从OPE中总共鉴定出5种暂定化合物，包括米罗苷、脱氧米罗苷、喜树碱、阿克那定和β - 谷甾醇。OPE对HepG2和SMMC - 7721细胞显示出强大的细胞抑制作用。OPE诱导这些细胞系的G2/M期细胞周期阻滞，增加凋亡，并增强ROS产生。此外，OPE通过降低MMP - 9和MMP - 2的表达对细胞迁移和侵袭具有显著抑制作用。而且，OPE显著抑制p65的磷酸化。

结论

我们的数据表明，OPE抑制肝癌细胞的增殖和迁移，这可能与抑制NF - κB通路有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/1de2ad2b2b9f/13020_2020_291_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/eca7a5f3800c/13020_2020_291_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/7cf8c5f12f19/13020_2020_291_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/633ed15c3f08/13020_2020_291_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/869aee3c504f/13020_2020_291_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/b252b4e9e781/13020_2020_291_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/1de2ad2b2b9f/13020_2020_291_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/eca7a5f3800c/13020_2020_291_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/7cf8c5f12f19/13020_2020_291_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/633ed15c3f08/13020_2020_291_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/869aee3c504f/13020_2020_291_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/b252b4e9e781/13020_2020_291_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59f0/6995237/1de2ad2b2b9f/13020_2020_291_Fig6_HTML.jpg

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