Plant Microbe Interaction Lab, Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi 221005, India.
Plant Microbe Interaction Lab, Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi 221005, India; Department of Botany, Pune University, Pune 411007, India.
Sci Total Environ. 2022 Aug 25;836:155550. doi: 10.1016/j.scitotenv.2022.155550. Epub 2022 May 1.
The main aim of the present work was to explore culturable bacteria and to develop potential microbial consortium as bio-inoculants for enhancing plant productivity, nutritional content, and soil health. For this study, we selected two bacterial strains e.g., Enterobacter hormaechei (BHUJPCS-15) and Brevundimonas naejangsanensis (BHUJPVCRS-1) based on plant growth-promoting activities We developed a consortium of both strains and estimated plant growth promotion (PGP) activity which recorded significant better production of Indole-3-acetic acid (IAA) (61.53 μg/ml), siderophore (12.66%), ammonia (98.66 μg/ml), phosphate solubilisation (942.64 μg/ml), potassium solubilisation, and antagonistic activity against Fusarium sp. than individual bacterial strains. Bacterial consortium (E. hormaechei + B. naejangsanensis) treatment significantly enhanced plant growth attributes, grain yields, nutritional content in plant and seed, followed by E. hormaechei as compared to control. Seed treated with consortium recorded a significant increase in available N P K, enzymes and microbial communities in soils. Microbiome analysis revealed that the dominance of bacterial group and its functional properties is directly correlated with plant growth attributes, nutrient content, soil N P K, and enzyme activity. The relative abundance of bacterial phyla Proteobacteria (98%) was dominantly recorded in all treatments. The microbiome of seed and soil, treated with consortium (E. hormaechei + B. naejangsanensis) showed high amount of diversity of bacterial phyla Verrucomicrobia, Firmicutes, Bacteroidetes, Acidobacteria, Chloroflexi, and Proteobacteria than E. hormaechei (Firmicutes, Bacteroidetes, Chloroflexi and Proteobacteria) and control (Firmicutes, Bacteroidetes and Proteobacteria). In soil, root and shoot, E. hormaechei treatment enriched ligninolytic, nitrogen fixation, cellulolytic, nitrate ammonification among other pathways. The main finding is that the consortium treated seed of chickpea recorded significant enhancement of plant growth attributes, productivity, nutritional content, and soil health as well as microbial colonization in soil and seed part.
本研究旨在探索可培养细菌，并开发潜在的微生物共生体作为生物接种剂，以提高植物生产力、营养含量和土壤健康。为此，我们选择了两株具有植物促生作用的细菌菌株，即肠杆菌（BHUJPCS-15）和黄单胞菌（BHUJPVCRS-1）。我们开发了这两种菌株的共生体，并评估了其植物促生（PGP）活性，结果表明，该共生体显著提高了吲哚-3-乙酸（IAA）的产量（61.53μg/ml）、铁载体（12.66%）、氨（98.66μg/ml）、磷酸盐溶解（942.64μg/ml）、钾溶解和对镰刀菌的拮抗活性，优于单一细菌菌株。与对照相比，细菌共生体（肠杆菌+黄单胞菌）处理显著提高了植物生长特性、籽粒产量、植物和种子中的营养含量。与对照相比，用共生体处理的种子在土壤中显著增加了有效氮、磷、钾、酶和微生物群落。微生物组分析表明，细菌群体的优势及其功能特性与植物生长特性、养分含量、土壤氮、磷、钾和酶活性直接相关。在所有处理中，变形菌门（Proteobacteria）的相对丰度（98%）最高。用共生体（肠杆菌+黄单胞菌）处理的种子和土壤的微生物组显示，与肠杆菌（Firmicutes、Bacteroidetes、Chloroflexi 和 Proteobacteria）和对照（Firmicutes、Bacteroidetes 和 Proteobacteria）相比，厚壁菌门（Verrucomicrobia）、Firmicutes、Bacteroidetes、Acidobacteria、Chloroflexi 和 Proteobacteria的细菌多样性更高。在土壤、根和茎中，肠杆菌处理富集了木质素分解、固氮、纤维素分解和硝酸盐氨化等途径。主要发现是，用共生体处理的鹰嘴豆种子显著提高了植物生长特性、生产力、营养含量和土壤健康，以及土壤和种子部分的微生物定植。
