Egan J E, Weber J L, Ballou W R, Hollingdale M R, Majarian W R, Gordon D M, Maloy W L, Hoffman S L, Wirtz R A, Schneider I
Science. 1987 Apr 24;236(4800):453-6. doi: 10.1126/science.3551073.
As part of a study of potential vaccines against malaria, the protective efficacy of sporozoite subunit vaccines was determined by using the Plasmodium berghei murine malaria model. Mice were immunized with recombinant DNA-produced or synthetic peptide-carrier subunit vaccines derived from the repetitive epitopes of the Plasmodium berghei circumsporozoite gene, or with radiation-attenuated sporozoites. Immunization with subunit vaccines elicited humoral responses that were equivalent to or greater than those elicited by irradiated sporozoites, yet the protection against sporozoite challenge induced by either of the subunit vaccines was far less than that achieved by immunization with attenuated sporozoites. Passive and adoptive transfer studies demonstrated that subunit vaccines elicited predominantly antibody-mediated protection that was easily overcome whereas irradiated sporozoites induced potent cell-mediated immunity that protected against high challenge doses of sporozoites. These studies indicate that new strategies designed to induce cellular immunity will be required for efficacious sporozoite vaccines.
作为一项针对疟疾潜在疫苗的研究的一部分，通过使用伯氏疟原虫小鼠疟疾模型来确定子孢子亚单位疫苗的保护效力。用源自伯氏疟原虫环子孢子基因重复表位的重组DNA产生的或合成肽载体亚单位疫苗，或用辐射减毒子孢子对小鼠进行免疫。用亚单位疫苗免疫引发的体液反应等同于或大于用辐照子孢子引发的体液反应，然而，两种亚单位疫苗中任一种所诱导的针对子孢子攻击的保护作用远小于用减毒子孢子免疫所实现的保护作用。被动和过继转移研究表明，亚单位疫苗主要引发易于被克服的抗体介导的保护作用，而辐照子孢子诱导有效的细胞介导免疫，可抵御高剂量子孢子的攻击。这些研究表明，有效的子孢子疫苗将需要设计新的策略来诱导细胞免疫。
