Okabe K, Malchesky P S, Nose Y
Tohoku J Exp Med. 1986 Dec;150(4):373-9. doi: 10.1620/tjem.150.373.
Mitochondrial function of the liver is one of the limiting factors in liver preservation. Nowadays, prostaglandin I2 (PGI2) is used as a cytoprotective agent in liver preservation without full understanding its mechanism involved. It is the objective of the present study to evaluate the protective effect of PGI2 on mitochondrial function of the rat liver during hypothermic preservation. Collins' solution was used as a preservation solution and PGI2 was added to it in some experimental groups. Both ischemic and non-ischemic livers were perfused with the preservation solution and preserved under simple hypothermia for 8 hr. Parameters of the mitochondrial function such as respiratory control, oxygen consumption rate in state 3 respiration, ADP/O ratio and the rate of ATP synthesis were decreased significantly after 8 hr hypothermic preservation, even if ischemic injury was not induced prior to preservation. ADP/O ratio, which represents the efficiency of oxidative phosphorylation in mitochondria, was improved significantly (p less than 0.01) when PGI2 was used as a cytoprotective agent. The rate of ATP synthesis, a parameter of energy producing reaction, showed a tendency to be increased by PGI2, but was not significant. It was concluded that hypothermic preservation of the rat liver is associated with the deterioration of the mitochondrial function and PGI2 has a favorable effect on the impaired ADP/O ratio of oxidative phosphorylation in mitochondria.
肝脏的线粒体功能是肝脏保存的限制因素之一。目前，前列腺素I2（PGI2）在肝脏保存中用作细胞保护剂，但对其作用机制尚未完全了解。本研究的目的是评估PGI2在低温保存期间对大鼠肝脏线粒体功能的保护作用。以柯林斯液作为保存液，在一些实验组中加入PGI2。将缺血和非缺血肝脏均用保存液灌注，并在单纯低温下保存8小时。低温保存8小时后，即使在保存前未诱导缺血损伤，线粒体功能参数如呼吸控制、状态3呼吸中的氧消耗率、ADP/O比值和ATP合成速率也显著降低。代表线粒体氧化磷酸化效率的ADP/O比值，当使用PGI2作为细胞保护剂时显著改善（p小于0.01）。作为能量产生反应参数的ATP合成速率有被PGI2升高的趋势，但不显著。得出的结论是，大鼠肝脏的低温保存与线粒体功能恶化有关，PGI2对线粒体氧化磷酸化受损的ADP/O比值有良好影响。
